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We developed a method, named GraFix, that considerably improves sample quality for structure determination by singleparticle electron cryomicroscopy (cryo-EM). GraFix uses a glycerol gradient centrifugation step in which the complexes are centrifuged into an increasing concentration of a chemical fixation reagent to prevent aggregation and to stabilize individual macromolecules. The method can ...
Current findings suggest a role for the angiotensin II (Ang II) signalling pathway in generation of reactive oxygen species and diabetes-induced cardiac complications. In this study we aimed to investigate the effect of angiotensin II type 1 (AT 1 ) receptor blockage on some antioxidant enzy mes such as glucose-6-phosphate dehydrogenase (G6PD), 6-phoshogluconate dehydrogenase (6PGD), glutathion...
Meal machine, experimental laboratory, status symbol, domestic prison, or the creative and spiritual heart of the home? Over the course of the past century no other room has been the focus of such intensive aesthetic and technological innovation, or as loaded with cultural significance. Kitchen design has been both a central concern of modernism and fundamental to our concept of modern life (Mo...
IN RECENT YEARS, THERE HAS BEEN A RAPID AND SIGNIFICANT PROGRESS IN UNDERSTANDING THE MOLECULAR BASIS OF THE CIRCADIAN RHYTHMICITY, AS SEVERAL CLOCK GENES HAVE BEEN CLONED IN MAMMALS. Studies with these genes focusing on loss of function have demonstrated abnormal sleep/wake length periods, disturbed entrainment, and loss of persistence of circadian rhythms. Current findings clearly indicate th...
Pathophysiology The thoracic duct develops from outgrowths of the jugular lymphatic sacs and the cisterna chyli. During embryonic life, bilateral thoracic lymphatic channels are present, each attached in the neck to the corresponding jugular sac. As development progresses, the upper third of the right duct and the lower two-thirds of the left duct involute and close. The wide variation in the f...
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