Background: High serum amylase is frequently found in perforated duodenal ulcer (PDU) ,the rise in serum amylase occurs in cases of perforated peptic ulcer partly as a result of increase leakage of pancreatic enzymes rich fluid from the perforation site with subsequent absorption by peritoneal lymphatics & partly due to damage of pancreases by digestive enzymes that spilled through the perforat...

AIM To investigate potential biomarkers for predicting postoperative pancreatic fistula (POPF) after pancreaticoduodenectomy (PD). METHODS We prospectively recruited 83 patients to this study. All patients underwent PD (Child's procedure) at the Division of Hepatobiliary and Pancreas Surgery at the First Bethune Hospital of Jilin University between June 2011 and April 2015. Data pertaining to...

We question the variations in serum concentrations of lipase and amylase during acute pancreatitis reported by Loft and Lu (1). In the caption for their Figure 3, the authors stated that the graphs of the two enzymes in a patient with acute pancreatitis demonstrated “characteristic changes with time.” Serum concentrations of both enzymes peaked on the fourth day after onset and still had not de...

This report describes a new procedure for serum amylase assay. A novel substrate, dyed amylopectin, is used that combines the advantages of saccharogenic and amyloclastic methods. Serum amylase hydrolyzes the dyed amylopectin into ethanol-soluble fragments, which are quantified colorimetrically after serum proteins and unhydrolyzed substrate are precipitated with alcoholic tannic acid. The subs...

We report a case of primary bronchogenic adenocarcinoma, complicated by pleural effusion, in which very high pleural amylase activity was found, whilst serum amylase was normal. Isoamylase determination showed a salivary-type amylase. Concerning the origin of this enzyme, ultrastructural study of the malignant cells obtained from the pleural fluid suggested a local amylase synthesis. The pathop...

Aim: To compare the amylase activity in the cervical mucus and serum during estrus in normal and repeat breeder cattle and find out importance in fertility. Materials and methods: Twenty cyclic crossbred cattle were divided into two groups: normal (n =10) and repeat breeder (n =10). Animals exhibiting spontaneous estrus, were artificially inseminated (AI) twice, as per AM-PM schedule along with...

We describe a case of exudative pleural effusion in a patient with mucinous pulmonary adenocarcinoma. Pleural fluid examination showed markedly raised amylase with normal serum amylase concentration. There was no clinical or radiological evidence of oesophageal rupture or pancreatitis. The case illustrates the importance of considering pulmonary malignancy in the context of amylase-rich pleural...

OBJECTIVE To examine the pancreatic exocrine insufficiency in patient with diabetes mellitus by estimating serum pancreatic amylase and lipase enzymes in healthy subjects and in type 1 and type 2 diabetic patients. METHODS The study was conducted on 20 normal healthy volunteers and 39 diabetic patients referred to Al-Isra Medical Laboratory, Amman, Jordan during the period from April - Novemb...

Background: The antioxidant defense in the pancreas is low because they are exposed to toxic substances daily. This study aimed to evaluate the ameliorating effect of Viola odorata flowers Extract (VOE) on the pancreas histology and function in Streptozotocin (STZ)-induced diabetic rats. Methods: Forty male rats were divided into five groups, consisting of controls; STZ; and STZ plus various d...

I describe a new kinetic enzymatic saccharogenic method for assaying alpha-amylase in human serum and urine. alpha-Amylase liberates maltose from starch. This is successively acted on by alpha-glucosidase, mutarotase, and glucose dehydrogenase. The resulting conversion of NAD+ to NADH, measured at 340 nm, during a 20-min incubation reflects amylase activity. Endogenous glucose is destroyed befo...