The pharmaceutical industry and most particularly the biopharmaceutical are demanding advancements in analytical techniques. field of proteomics peptide mapping needs sensitive analysis sophisticated instrumentation, which hyphenated techniques to be explored. In addition effective detection characterization, Due efficient selective separation, predominant focus has been drawn capillary electro...

Characterization of blood proteins for clinical purposes was initiated in the middle of the nineteenth century and serum protein electrophoresis was first developed in the 1930s by a Swedish biochemist Arne Tiselius. Today, clinical analysis of human serum proteins by the electrophoretic method that evolved from the original fluid method of Tiselius, through paper electrophoresis, cellulose ace...

Capillary zone electrophoresis (CZE) and immuno-subtraction electrophoresis (ISE) were evaluated for ability to detect and immunotype monoclonal proteins, compared with agarose gel electrophoresis (AGE) and immunofixation electrophoresis (IFE), respectively. Six hundred seventeen serum samples were analyzed with CZE and AGE to determine sensitivity and specificity in detecting IFE-confirmed mon...

BACKGROUND Autosomal forms of Alport syndrome represent 20% of all patients (15% recessive and 5% dominant). They are caused by mutations in the COL4A3 and COL4A4 genes, which encode a-3 and a-4 collagen IV chains of the glomerular basement membrane, cochlea and eye. Thin basement membrane nephropathy may affect up to 1% of the population. The pattern of inheritance in the 40% of cases is the s...