Canine coronavirus (CCV) and canine parvovirus type 2 (CPV-2) are the causative agents of gastroenteritis in dogs. Seventy fecal samples from dogs with signs of gastroenteritis (vomiting and diarrhea), twenty-five fecal samples from healthy dogs and one CPV-2 vaccine strain were amplified by semi-nested polymerase chain reaction (PCR) and semi-nested reverse transcriptase polymerase chain react...

Assay Manufacturer Method Detection limit or dynamic range (IU/mL) Qualitative HCV RNA dection assays AmplicorTM HCV 2.0 Roche Molecular System Manual RT-PCR 50 Cobas AmplicorTM HCV 2.0 Roche Molecular System Semi-automated RT-PCR 50 VersantTM HCV Siemens Health Care Diagnostics Semi-automated TMA 10 Quantitative HCV RNA detection assays AmplicorTM HCV Monitor Roche Molecular System Manual RT-P...

BACKGROUND The main goal of this study was to evaluate the diagnostic efficacy of melanoma-associated antigen (MAGE) A1-6 reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) of bronchial washing fluid for the detection of lung cancer invisible by bronchoscopy. PATIENTS AND METHODS To determine the expression of MAGE A1-6 gene in 75 lung carcinomas diagnosed by conventional ...

We describe the evaluation of a nested reverse transcriptase PCR (RT-PCR) procedure for the detection of small round-structured viruses (SRSVs) in molluscan shellfish and the application of this assay for the detection of SRSVs in commercially produced shellfish and in shellfish implicated in outbreaks of gastroenteritis. The range of virus strains detected and the sensitivity of detection were...

sodium/proton exchangers (nhx) are key players in plant responses to salinity and have a central role in establishing ion homeostasis. nhxs can be localized in tonoplast or plasma membranes, where they exchange sodium ions for protons, resulting in the removal of ions from the cytosol into vacuole or extracellular spaces. in the present study, the expression pattern of the gene encoding na+/h+ ...

introduction: calcium/calmodulin-dependent protein kinase ii (camkii) is highly expressed in the hippocampus, which has a pivotal role in reward-related memories and morphine dependence. methods: in the present study, morphine tolerance was induced in male wistar rats by 7 days repeated morphine injections once daily, and then gene expression profile of α-isoform of camkii (camkiiα) in the hipp...