AIM To obtain individual Y-short tandem repeat (STR) profiles in a multi-suspect sexual assault case. Methods. We used laser cut microdissection to capture the single sperm cell in the multi-contributor semen sample, combined with the low volume polymerase chain reaction (LV-PCR) method to genotype the single sperm cell profiles using the Yfiler(®) kit. Consensus DNA profiles were generated fro...

in 30–50% of sub-fertile couples the male partner has poor semen quality, either because of low sperm count, poorly motile sperm or sperm with abnormal size and shape (morphology). This may result from inherent genetic factors, poor pre-natal testicular development or adult exposure to gonadotoxic agents. There are few proven therapies to improve semen quality. Therefore, the number and quality...

STUDY QUESTION Does paternal age affect semen quality and reproductive outcomes in oocyte donor cycles with ICSI? SUMMARY ANSWER Paternal age is associated with a decrease in sperm quality, however it does not affect either pregnancy or live birth rates in reproductive treatments when the oocytes come from donors <36 years old and ICSI is used. WHAT IS KNOWN ALREADY The weight of evidence s...

OBJECTIVE To examine fecundability trends among sperm donors. DESIGN Retrospective analysis. SETTING University-based sperm bank and donor insemination program. PATIENT(S) Sperm donors and recipients. INTERVENTION(S) A group of recipients underwent IUI with cryopreserved donor sperm. Fecundability was calculated for 20 sperm donors over 800 insemination cycles. MAIN OUTCOME MEASURE(S)...

Effect of two antioxidants (Taurine and Manganese) in extender on different spermparameters (sperm motility, live sperm percentage, membrane acrosome integrity) Sahiwal bullswas evaluated. Semen was collected twice a week for 8 weeks from six adult, regular donor andclinically healthy bulls. A total 96 ejaculates were pooled after initialevaluation. The semen samples diluted at with tris-citric...

This study investigates the motivations, views and experiences of semen donors willing to have contact with their offspring. An online questionnaire for semen donors was posted by the US-based Donor Sibling Registry in 2009. A total of 164 respondents who had previously been donors completed the questionnaire, which consisted of 45 open and closed questions covering motivations for donating, he...

BACKGROUND The present study was conducted to evaluate seasonal variability in the quality of pre- and post-thaw semen parameters among sperm bank donors. METHODS The first two consecutive ejaculates during the months March (spring, 92 males), June (summer, 97 males), September (autumn, 81 males) and December (winter, 97 males) were analysed. A comparison was made between sperm parameters fro...

BACKGROUND One major limitation in the use of sperm DNA fragmentation as measured by the TdT (terminal deoxynucleotidyl transferase)-mediated dUTP nick-end labelling (TUNEL) assay is the paucity of solid data on the stability of this parameter. METHODS The objective of our study was to evaluate variations in the degree of sperm DNA fragmentation, as measured by the TUNEL assay, over a 6 month...

Human papillomaviruses (HPV) may cause sexually transmitted disease. High-risk types of HPV are involved in the development of cervical cell dysplasia, whereas low-risk types may cause genital condyloma. Despite the association between HPV and cancer, donor sperm need not be tested for HPV according to European regulations. Consequently, the potential health risk of HPV transmission by donor ba...

The extent to which certain parameters can influence pregnancy rates after intrauterine insemination with frozen donor semen was examined prospectively. Between July 2011 and September 2015, 402 women received 1264 IUI cycles with frozen donor semen in a tertiary referral infertility centre. A case report form was used to collect data prospectively. The primary outcome measure was clinical preg...