A novel rotor was constructed to allow for the seeding of porous scaffolds via centrifugal force. Using cell seeding times of 10 min, this method placed significantly (roughly 3-fold) more cells into poly(glycolic acid) scaffolds than 24 h of spinner flask seeding or static seeding. There were no significant differences in the mitochondrial activity per cell between the 3 seeding methods. Cell ...

Spongiform encephalopathies are believed to be transmitted by self-perpetuating conformational conversion of the prion protein. It was shown recently that fundamental aspects of mammalian prion propagation can be reproduced in vitro in a seeded fibrillization of the recombinant prion protein variant Y145Stop (PrP23-144). Here we demonstrate that PrP23-144 amyloids from different species adopt d...

In this paper, we explore the establishment and evolution of the empirical correlation between black hole mass (MBH) and velocity dispersion (σ) with redshift. We trace the growth and accretion history of massive black holes (MBHs) starting from high redshift seeds that are planted via physically motivated prescriptions. Two seeding models are explored in this work: ‘light seeds’, derived from ...

The glutamine/asparagine (Q/N)-rich yeast prion protein Sup35 has a low intrinsic propensity to spontaneously self-assemble into ordered, beta-sheet-rich amyloid fibrils. In yeast cells, de novo formation of Sup35 aggregates is greatly facilitated by high protein concentrations and the presence of preformed Q/N-rich protein aggregates that template Sup35 polymerization. Here, we have investigat...

In this paper we propose a class of propagation models for multiple competing products over a social network. We consider two propagation models: the social conversion mechanism and the self conversion mechanism, corresponding respectively to the endogenous and exogenous factors. A novel concept, the product-conversion graph, is proposed to characterize the interplay among competing products. A...

In this paper, we explore the establishment and evolution of the empirical correlation between black hole mass (MBH) and velocity dispersion (σ ) with redshift. We trace the growth and accretion history of massive black holes (MBHs) starting from high-redshift seeds that are planted via physically motivated prescriptions. Two seeding models are explored in this work: ‘light seeds’, derived from...

Prions are protein conformations that "self-seed" the misfolding of their non-prion iso-forms into prion, often amyloid, conformations. The most famous prion is the mammalian PrP protein that in its prion form causes transmissible spongiform encephalopathy. Curiously there can be distinct conformational differences even between prions of the same protein propagated in the same host species. The...

Single crystals of poly(ethylene glycol) (PEG)-b-polystyrene (PS), PEG-b-poly(methyl methacrylate) (PMMA), PEG-b-polycaprolactone (PCL), and polyaniline (PANI)-b-PEG-b-PANI were developed from dilute solutions and thin molten films using self-seeding methodology. The PS and PMMA grafted chains were categorized in disordered nano-brushes; however, the PCL and PANI ones were grouped in ordere...