A novel acid proteinase (Tropiase) was isolated from Candida tropicalis IFO 0589 by DE52-cellulose, and DEAE-Cosmogel column chromatographies. The purified tropiase gave a single band on disc polyacrylamide gel electrophoresis, isoelectric focusing and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The enzyme preparation had a molecular weight of 23,900, isoelectric point of p...

Abbreviations: PTMs=post-translational modifi cations; PPIs=protein-protein interactions; SDS-PAGE= sodium dodecyl sulfate-polyacrylamide gel electrophoresis; 2D-PAGE=two-dimensional polyacrylamide gel electrophoresis; MS=mass spectrometry; ESI=electrospray ionization; MALDI=Matrix Assisted Laser Desorption Ionization; ESI-MS=electrospray ionization mass spectrometry; MALDI-MS=MALDI mass spectr...

Mitogen-stimulated mononuclear blood cells produce differentiation inducing factors (DIFs) for the promyelocytic cell line HL-60. We report that DIF is produced constitutively by a malignant T lymphocyte line HUT-102. DIF was purified 7.000-fold from HUT-i 02 conditioned media by utilizing ion-exchange chromatography with DEAE-Sepharose, gel chromatography, Blue-Sepharose chromatography. and pr...

The protein analysis of Pasteurella multocida organism was performed by sodium dodycyle sulphate polyacrylamide gel electrophoresis (SDS-PAGE) technique using a high pH discontinous buffer system. Six isolates of the Pasteurella multocida organism were confirmed by Gram’s staining and different biochemical tests. In the protein fractions of purified Pasteurella multocida strains, seven polypept...

1. Protein Separation Methods The following is a quick review of some common methods used for protein separation: SDS-PAGE (SDS-polyacrylamide gel electrophoresis) separates proteins mainly on the basis of molecular weight as opposed to charge (which is ‘swamped out’ by the excess of protein-bound SDS) or folding (proteins are largely denatured in SDS). In the ideal picture, the distance migrat...

We have developed an electrophoretic method to determine the size of a protein—DNA complex using the same binding and electrophoresis conditions as in an electrophoretic mobility shift assay (EMSA). The method is an adaptation of that of Ferguson (1), originally developed to determine the size of native proteins in non-denaturing gel systems. In SDS polyacrylamide gel electrophoresis, associati...

Abstract Genetic distances among different chickpea varieties and evaluation of their free amino acid profiles were determined on the basis Sodium dodecyle sulphate polyacrylamide gels electrophoresis (SDS-PAGE). Total soluble proteins resolved 10% SDS Polyacrylamide gel. Low variability in tested was observed. Dendogram based electrophoretic data clustered genotypes into 2 groups. The results ...

we have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-de). this modified version of 2-de is not only simpler and easier but also faster than all the currently available methods. in this method, isoelectric focusing is carried out in the first dimension using a vertical sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-...

Hydropericardium syndrome (HPS) agent was purified by sucrose gradient centrifugation. The polypeptides of HPS agent were separated through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and identified by known protein markers. Eight polypeptides having molecular weights ranging from 119.0 to 15.7 KDa were observed. The nucleic acid of HPS agent was extracted and determin...