1 Principal Investigator of Horticulture and Curator, USDA Naitonal Clonal Germplasm Repository for Asimina species, Atwood Research Facility, Kentucky State University, Frankfort, KY 40601-2355. To whom reprint requests should be addressed. 2 Co-Investigator of Horticulture, Atwood Research Facility, Kentucky State University, Frankfort, KY 40601-2355 Abstract The pawpaw [Asimina triloba (L.) ...

The objectives of this research are to develop the grafting robots and an acclimatization chamber for watermelon, and tomato seedlings. For watermelon seedlings, a top plug-in robotic grafting system has been developed, which is applicable for grafting a scion into a mature rootstock before the scion’s cotyledons spread. It thus makes the scion to be very tender and greatly different from the r...

Citrus roots have rare root hairs and thus heavily depend on arbuscular mycorrhizal fungi (AMF) for mineral nutrient uptake. However, the AMF community structure of citrus is largely unknown. By using 454-pyrosequencing of 18S rRNA gene fragment, we investigated the genetic diversity of AMF colonizing citrus roots, and evaluated the impact of habitats and rootstock and scion genotypes on the AM...

* The extreme phenotype of zinc (Zn) hyperaccumulation, which is found in several Brassicaceae species, is determined by mechanisms that promote elevated Zn tolerance and high Zn accumulation in shoots. * We used reciprocal grafting between a Zn hyperaccumulator, Thlaspi caerulescens, and a Zn nonaccumulator, Thlaspi perfoliatum, to determine the relative importance of roots and shoots in Zn hy...

We investigated the hydraulic architecture of young olive trees either self-rooted or grafted on rootstocks with contrasting size-controlling potential. Clones of Olea europea L. (Olive) cv 'Leccino' inducing vigorous scion growth (Leccino 'Minerva', LM) or scion dwarfing (Leccino 'Dwarf', LD) were studied in different scion/rootstock combinations (LD, LM, LD/LD, LM/LM, LD/LM and LM/LD). Shoots...

Although grafting is broadly used in the production of crops, no information is available about the proteins involved in vascular connections between rootstock and scion. Similarly, proteome changes under the light intensities widely used for grafted seedlings are of practical use. The objective of this study was to determine the proteome of vascular connections using watermelon (Citrullus vulg...

Using grafting procedures, we investigated the transmission of co-suppression of nitrate reductase and nitrite reductase host genes and transgenes and of post-transcriptional silencing of a uidA transgene encoding glucuronidase in tobacco. We demonstrate that silencing is transmitted with 100% efficiency from silenced stocks to non-silenced scions expressing the corresponding transgene. Transmi...

Reciprocal cleft and pistillate floral bud grafts were made between parthenocarpic ;Fertilla' and nonparthenocarpic ;MSU 713-5' cucumber (Cucumis sativus L.) lines to localize the site for stimulation of parthenocarpic fruit set. No fruit set on ;MSU 713-5' controls, scion grafted to ;Fertilla,' or rootstock with ;Fertilla' as the scion. ;Fertilla' controls, rootstock, and scions all produced p...

hazelnuts are usually propagated by suckers or layering. however, other methods of propagation have been tested with variable results. grafting is a method that has a few advantages such as fast multiplication rate, earlier fruiting and reducing sucker removal cost on specific rootstocks. unfortunately, grafting is difficult due to slow formation of callus in hazelnuts. here, we report the hypo...

The research has been done for assessment of effects of different concentrations of Indole-3-Butyric Acid (IBA) on rhizogenesis of scions of C. Phlomidis in 2010.  The research has been done on Randomized Complete-blocks for five replications and three treatments with zero, 2000ppm and 3000pmm concentration. Different characters such as number of root of each scion, length of root, length of ne...