Shoot multiplication of Bupleurum distichophyllum was achieved from the nodal and shoot tip explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response was from axillary shoots and the highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of axillary shoot proliferation was fou...

The movement of abscisic acid in the cotton explant was investigated by bioassay and radioisotope techniques. The rate of movement was 20 to 30 millimeters per hour with most of the abscisic acid moving unchanged through the explant into the basal agar. The rate of movement was the same through the abscission zone as through petiole tissue. Patterns of accumulation and metabolic products are di...

In micropropagation of lily, preferably bulblets should be produced: Because bulblets are compact and robust, they are much easier to handle and to plant in soil than shoots. In this review, the various factors that determine bulblet growth in vitro are discussed. Gibberellins, jasmonates (JA) and abscisic acid (ABA) are the major identified plant growth regulators (PGRs) for storage organ form...

A novel method of shoot regeneration from immature floral buds along with inflorescence axis in C. borivilianum, a rare medicinal herb is described. Using this explant, axenic cultures were established with very less contamination (10%). MS medium with 2 mg l(-1) kinetin and 0.1 mg l(-1) 2, 4-dichlorophenoxyacetic acid proved to be the best for multiple shoot induction. Maximum number (35) of s...

An efficient regeneration system through somatic embryogenesis was developed for date palm cv. Najda. Adventitious bud and proximal leaf segments cultured on Murashige and Skoog (MS) medium supplemented with various combinations of auxins and cytokinins induced embryogenesis after at least 6 months of culture. Somatic embryogenesis induction seemed correlated with the type of the explant, the i...

To optimize conditions for micropropagating Galanthus transcaucasicus Fomin, the effect of explant type, different concentrations of sucrose, type and different concentrations of auxin were examined on bulblet production. For the induction of bulblets, three types of bulb scales were usesd as explants. A two-step sterilization procedure was applied. Assessments were made after incubation for 20...

Mouse submandibular salivary gland cells were grown in primary explant culture. After an initial period of degeneration within the explant, surviving epithelial cells proliferated rapidly and duct-like structures recolonized the explant. Autoradiographic studies showed that a peak of DNA synthesis occurred after 4 days in vitro and that proliferation was enhanced by insulin and hydrocortisone. ...

Effect of Explant, Type and Concentration Hormone on Callus Induction Regeneration two Iranian Sesame (Sesamum indicum L.) Cultivars

OBJECTIVES Clinical trials of spinal cord stimulation (SCS) have largely focused on conversion from trial to permanent SCS and the first years after implant. This study evaluates the association of type of SCS and patient characteristics with longer-term therapy-related explants. MATERIALS AND METHODS Implanting centers in three European countries conducted a retrospective chart review of SCS...

Micropropagation of Kainth using nodal explants was carried out in tissue culture laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2011-13. The effect of various media {1/2 MS (M1), MS (M2) and WPM (M3)} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was studied. Per cent necrotic culture was found to be influenced by...