The aim of this study is to investigate the expression of sarcosine metabolism related proteins according to androgen receptor (AR) and HER-2 status in estrogen receptor (ER) negative breast cancer and to analyze its clinical implications. Tissue microarray was constructed for a total of 334 cases of ER negative breast cancer. Immunohistochemical stain was conducted for sarcosine metabolism rel...

The pathway for the degradation of glyphosate (N-phosphonomethylglycine) by Pseudomonas sp. PG2982 has been determined by using metabolic radiolabeling experiments. Radiorespirometry experiments utilizing [3-14C]glyphosate revealed that approximately 50 to 59% of the C-3 carbon was oxidized to CO2. Fractionation of stationary-phase cells labeled with [3-14C]glyphosate revealed that from 45 to 4...

In the system sarcosine + L-tartaric acid + H2O we identified several new phases in addition to the previously known species sarcosinium hydrogen L-tartrate (I). Two of them (phases (II) and (III)) were obtained in the form of single crystals. Their crystal and molecular structures were determined. Both phases (II) and (III) have 2:1 composition and contain a zwitter-ionic sarcosine, a sarcosin...

The coupled feeding of sarcosine (N-methylglycine) and benzoic acid, like the coupled feeding of glycine and benzoic acid, results in the excretion of hippuric acid at a rate greater than that when benzoic acid is fed alone (1). When sarcosine containing an excess of N16 is fed to rats, the isotope content of the amino acids of the tissues is almost identical with that of animals fed glycine co...

The oxidation of sarcosine (N-methylglycine) by purified rat liver sarcosine dehydrogenase is known to require the participation of a flavin adenine dinucleotide-dependent protein, the electron-transferring flavoprotein, for efficient transfer of electrons between reduced dehydrogenase and 2,6-dichlorophenolindophenol (l), or cytochrome c (a), or cytochrome b (2). On the basis of spectral evide...

The oxidation of sarcosine (N-methylglycine) by purified rat liver sarcosine dehydrogenase is known to require the participation of a flavin adenine dinucleotide-dependent protein, the electron-transferring flavoprotein, for efficient transfer of electrons between reduced dehydrogenase and 2,6-dichlorophenolindophenol (l), or cytochrome c (a), or cytochrome b (2). On the basis of spectral evide...

Formaldehyde was shown to be a normal metabolite when Handler, Bernheim, and Klein (1) found sarcosine oxidase which catalyzes the oxidative demethylation of sarcosine to glycine and formaldehyde. Sakami (2), Welsh and Sakami (3), and Stekol et al. (4) have recently presented evidence that formate may provide the animal with necessary methyl groups. It was therefore of interest to investigate t...

When it was discovered in this laboratory that sarcosine and dimethylglycine are oxidized to formaldehyde by riboflavin phosphate and riboflavin in the absence of protein,1 this observation was pursued, not only because of our interest in the enzymatic oxidation of these substrates, but also because of its implications regarding the origin of biochemical reactions. Subsequent studies to be desc...