expression of htlv-i p19 protein in an escherichia coli expression system always leads to the formation of inclusion body. solubilisation and refolding of the inclusion bodies is complex, time consuming and difficult during large-scale preparation. this study aimed to express and purify a soluble form of recombinant htlv-i p19 protein in an e. coli expression system. the synthetic dna encoding ...

selection and use of tolerant grapevine as rootstock or own-rooted vines and finding indexes for screening of resistant genotypes have especial importance. to identify the salt tolerant grapevine genotypes, effects of nacl concentrations (0, 50, 100 and 150 mm) on root length, root and shoot dry weight, prolin, total soluble solid, rwc, level of salt injury, leaf concentration of k, na, cl and ...

When rough microsomes are subjected to limited proteolysis and high salt, a soluble fraction can be separated from the membrane. Neither fraction alone is capable of vectorially translocating nascent peptides. When the soluble extract is recombined with the residual membrane fraction, translocating activity is restored. Standard biochemical techniques were used to identify and characterize the ...

Macauba palm (Acrocomia aculeata L.) fruits are emerging sources to produce high-quality oils from pulp and kernels. Yet, the protein-rich press cake kernels remains underutilized, mainly due lacking knowledge of protein composition properties. Therefore, our aim was characterize main fractions The globulins were major fraction (58.5% storage proteins), which further separated into 11 S 7 globu...

Wheat is affected by various biotic and abiotic stresses, especially salinity, which reduces the growth yield drastically. With this view, an experiment was conducted to observe genotypic differences in agro-morphological, yield, biochemical responses salinity. Experimental variables consisted of five salt-tolerant genotypes (G 13, G 20-1, 9, 22, 20-2), one susceptible genotype 24) standard che...

Salt stress is one of the most serious abiotic stresses limiting plant growth and development. Calcium as an essential nutrient element important signaling molecule plays role in ameliorating adverse effect salinity on plants. This study aimed to investigate impact exogenous calcium improving salt tolerance Tartary buckwheat cultivars, cv. Xinong9920 (salt-tolerant) Xinong9909 (salt-sensitive)....