A novel salicylate-degrading Streptomyces sp., strain WA46, was identified by UV fluorescence on solid minimal medium containing salicylate; trace amounts of gentisate were detected by high-pressure liquid chromatography when strain WA46 was grown with salicylate. PCR amplification of WA46 DNA with degenerate primers for gentisate 1,2-dioxygenase (GDO) genes produced an amplicon of the expected...

Salicylate, one of the most widely used drugs, produces at repetitive high doses reversible tinnitus and hearing loss. Neural correlates of hearing loss have long been established, whereas they remain elusive for tinnitus. The average spectrum of electrophysiological cochleoneural activity (ASECA), a measure of spontaneous auditory nerve activity, was monitored in guinea pigs over weeks of sali...

Previous investigations in this Laboratory (Hetzel, Charnock & Lander, 1959; Charnock, Lockett & Hetzel, 1962) have shown that the administration of therapeutic doses of salicylate drugs to both man and rats disturbs the normal electrolyte balance in addition to increasing the metabolic rate. The chronic administration of both sodium salicylate and potassium salicylate to rats produces a signif...

At antipyretic and analgesic doses, salicylate has no antiplatelet or anti-inflammatory effects, unlike typical inhibitors of the prostaglandin H synthases (PGHSs). We demonstrated that salicylate inhibits PGHS-1 and -2 with a potency inversely related to ambient hydroperoxide concentrations. Salicylate inhibition of PGHS-1 was prevented by 12-hydroperoxyeicosatetraenoic acid (12-HPETE). Increa...

WIDE VARIETY OF METHODS are available for the determination of salicylate in body fluids and tissues. These range from the spot test type used at the patients’ bedside to the organic solvent partition methods employed in more exact studies. In recent years, the method of Brodie et al. (1) involving extraction with ethylene chloride has gained widespread acceptance. The method commonly employs 1...

Salicylate inhibition of the secondary antibody response initiated in vitro on day 0 has been studied in cultures of rabbit lymph node fragments. Levels of 1.25 to 1.5 mM (0.20 to 0.24 mg/ml) sodium salicylate present in serum-free medium throughout an 18- or 21-day culture period completely inhibit the secondary response. This inhibition is largely accomplished by the drug's action during the ...

High dose sodium salicylate causes moderate, reversible hearing loss and tinnitus. Salicylate-induced hearing loss is believed to arise from a reduction in the electromotile response of outer hair cells (OHCs) and/or reduction of KCNQ4 potassium currents in OHCs, which decreases the driving force for the transduction current. Therefore, enhancing OHC potassium currents could potentially prevent...

Electrochemical sensing of methyl salicylate, a key plant volatile has been achieved using a gold nanoparticle (AuNP) modified screen printed carbon electrode (SPCE). The electrochemical response of planar gold electrodes, SPCE and AuNP-SPCE in alkaline electrolyte in the presence and absence of methyl salicylate were studied to understand the amperometric response of various electrochemical re...

Salicylate intoxication is a very common problem in everyday clinical practice. We describe a new case of salicylate-induced reversible Fanconi's syndrome in a 28-year-old Cypriot male who ingested 50 g of acetylsalicylic acid with suicidal intent. To our knowledge, this is the second case ever described. It is suggested that clinicians should be aware of this salicylate intoxication-induced co...