نتایج جستجو برای: rt pcr nested pcr

تعداد نتایج: 222624  

2001
Mario Menschikowski Margot Vogel Rolf Eckey Gerd Dinnebier Werner Jaross

In the present study a protocol of in situ reverse transcriptase-nested polymerase chain reaction (in situ RT-nested PCR) was examined based on the following modifications. (i) To exclude false positive signals caused by "DNA repair mechanisms" and "endogenous priming", a two-step PCR was applied after reverse transcription. The first step was performed in the presence of extrinsic primers and ...

Farzaneh Sabahi, Fatemeh Zali, Hooman Kaghazian, Mahdi Paryan, Manoochehr Makvandi, Mostafa Ghaderi, Seyed Dawood Mousavi-Nasab, Siamak Mirab Samiee,

Background: Human rotavirus (HRV) is the causative agent of severe gastroenteritis in children and responsible for two million hospitalizations and more than a half-million deaths annually. Sequence characteristics of the gene segments encoding the VP7 and VP4 proteins are used for the genotype classification of rotavirus. A wide variety of molecular methods are available, mainly based on rever...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 2004
Vassiliki Bozionellou Dimitris Mavroudis Maria Perraki Savvas Papadopoulos Stella Apostolaki Efstathios Stathopoulos Aliki Stathopoulou Evi Lianidou Vassilis Georgoulias

PURPOSE The detection of disseminated occult breast cancer cells in peripheral blood and bone marrow is associated with poor prognosis. Since a high proportion of these cells express the HER-2 receptor, we evaluated the effectiveness of the anti-HER-2 antibody trastuzumab (Herceptin) administration to eliminate them. EXPERIMENTAL DESIGN Thirty patients with prior chemotherapy exposure were re...

2014
Mani Revathy Kulandai Lily Therese Radhakishnan Bagyalakshmi Chokaliingam Chandrasekar Suria Kumar Hajib N. Madhavan

BACKGROUND & OBJECTIVES As there are no standard laboratory techniques for the rapid detection of Pneumocystis jirovecii in India, this study was undertaken to evaluate and establish an optimal and rapid technique for the detection of P. jirovecii by comparing three different techniques - staining technique, application of a real time polymerase chain reaction (RT-PCR) targeting kex 1 gene and ...

2005
D. Mittal N. Jindal

During the period from July 2002 to June 2003, infectious bursal disease (IBD) was suspected in 101 commercial flocks of broiler chickens on the basis of clinical and post mortem findings in some districts of the Haryana state, India. Bursal samples were collected randomly from 20 flocks for the detection of infectious bursal disease virus (IBDV) by reverse transcriptionpolymerase chain reactio...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 1997
Y Zhang C D Zippe F Van Lente E A Klein M K Gupta

Accurate staging is an important issue in managing patients with prostate cancer. Current staging modalities are poor predictors for locally advanced disease. In the present study, we investigated the role of a peripheral blood-based, nested reverse transcription-PCR (RT-PCR) for prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSM) in prostate cancer staging. Our nested ...

Journal: :Journal of virological methods 2005
Antonio Olmos Edson Bertolini Maite Gil Mariano Cambra

A TaqMan real-time RT-PCR was developed to detect and quantify RNA-targets from the non-circulative, non-persistently transmitted Plum pox virus (PPV) in individual fresh or aphids captured previously and squashed on paper. Reliable quantitation ranged from 40 up to 4 x 10(8) copies of control transcripts. This technique was applied successfully to plant material and to individual PPV vector (M...

Journal: :iranian biomedical journal 0
اسماعیل بابایی esmaeil babaei سیدجواد مولی seyed javad mowla شمس شریعت تربقان shams shariat torbaghan مجتبی عمادی بایگی mojtaba emadi bayegi

osteosarcoma is a relatively uncommon malignancy however, it is the most common form of primary malignant bone tumors in human. diagnosis and prognosis of patients with osteosarcoma is limited to clinico-radiopathological parameters, whereas molecular markers of tumor aggression have been poorly identified. survivin, an inhibitor of apoptosis (iap), is unique for its expression in human tumors ...

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