Phosphorylation of rat liver RNA polymerase I occurred when intact rat liver nuclei were incubated with [gamma32P]ATP and N6,O2' dibutyryl cyclic 3':5'-AMP. In addition, partially purified RNA polymerase I could be phosphorylated in vitro by an endogenous protein kinase. Phosphorylation by either method was followed by extensive purification of the enzyme. This revealed that 32P remained bound ...

A rapid and simple method for the isolation and purification of dsRNA is presented. The crucial step of this method is the extraction of proteins and DNA with acid phenol. After the extraction, only RNA is left in the aqueous phase. ssRNA contamination of the RNA preparation can be greatly reduced when ammonium sulfate is present during the extraction.

A procedure has been developed which facilitates the detection of measles virus RNA sequences in human brains. The procedure involves isolating subviral components (nucleocapsids) from brain tissues prior to RNA purification, followed by hybridization of these RNAs to cDNA synthesized from measles virus 50 S RNA template. Using these techniques we were able to obtain an RNA fraction which was m...

Avian myeloblastosis virus produced in vitro by myeloblastic cells in suspension was labeled with 32P and 14Camino acids. After partial purification of the virus, and pronase digestion, an RNA was extracted by phenol into the aqueous phase. Glycerol gradient centrifugation showed that the 32P-labeled RNA con tained 2 distinct components: a high molecular weight fraction sedimenting at about 67 ...

Avian myeloblastosis virus produced in vitro by myeloblastic cells in suspension was labeled with 32P and 14Camino acids. After partial purification of the virus, and pronase digestion, an RNA was extracted by phenol into the aqueous phase. Glycerol gradient centrifugation showed that the 32P-labeled RNA con tained 2 distinct components: a high molecular weight fraction sedimenting at about 67 ...