have been prepared and studied for possible incorporation into polynucleotides. Arab inosylcytosine mono-, di-, and triphosphates were synthesized by both chemical and enzymatic methods. Labeled and unlabeled arabinosylcytosine nucleotides were tested for their capacity to serve as substrates for purified polynucleotide phosphorylase, deoxyribonucleic acid-dependent ribonucleic acid polymerase,...

Submit Manuscript | http://medcraveonline.com Abbreviations: PBMC: Peripheral Blood Mononuclear Cells; TME: Tumor Microenvironment; FFPE: Formalin-Fixed ParaffinEmbedded; OCT: Optimal Cutting Media; RNA: Ribonucleic Acid; DNA: Deoxy Ribonucleic Acid; TCR: T Cell Receptor; TIL: Tumor Infiltrating Lymphocytes; IHC: Immunohistochemistry; Cytof: For Cytometry by Time of Flight; Elispo: Enzyme Linke...

It has become evident that several enzymatic reactions exist which lead to the incorporation of the mononucleotide moiety of the ribonucleoside triphosphates into ribonucleic acid (1). The first of these enzymes to be characterized catalyzes the addition of cytosine 5’-phosphate and adenosine 5’-phosphate to the nucleoside terminal end of soluble ribonucleic acid (2,3). The product of this reac...

The average cellular content of ribonucleic acid and protein was determined in cultures of Saccharomyces cerevisiae growing exponentially at different rates in a variety of media. Estimations of the proportion of total cellular ribonucleic acid that is made up of ribosomal ribonucleic acid were used to calculate the average number of ribosomes per cell at the different growth rates. The fractio...