Bacterial strains and growth conditions. Fast-growing soybean Rhizobium strains USDA 191, USDA 192, USDA 193, USDA 194, USDA 201, USDA 205, USDA 206, USDA 208, USDA 214, USDA 217, and USDA 257 were isolated from soil or nodules collected in the PRC (20). Slow-growing soybean rhizobium strains PRC 005, PRC 74, PRC 113-2, PRC121-6, PRC 2031, and PRC B15, were oHtained from T. S. Hu, Institute of ...

Pyruvic acid and O-acetyl groups are the major noncarbohydrate substituents in exopolysaccharides (EPS) produced by fast-growing species of Rhizobium. EPS substituent variations were observed among strains of the same species. The amounts of these substituents also varied with culture age; pyruvic acid increased in the EPS of all four species, whereas O-acetyl increased in Rhizobium trifolii an...

Salinity-induced changes in the protein profiles in Rhizobium sp. exhibited alterations in proteins, which either showed an enhanced rate of synthesis or a decline in the levels as compared with controls. The in vitro and in vivo Rhizobium of Cajanus Cajan plant produced high amount of protein from halophillic glyphosate (Hg) as compared to the Normal (N) plant. The proteomic diversity contribu...

background and objectives: soybean is one of the plants that need large amounts of nitrogen for grain yield production. todays bio-fertilizers are considered as a best replacement for chemical nitrogen to increase soil fertility and sustained agricultural production. meanwhile, the use of soybean symbiotic bacteria and nitroxin, which contain the most effective nitrogen-fixing bacteria of the g...

To study the influence of rhizobium inoculation on growth and yield of groundnut (Arachis hypogaea) cultivars, an experiment was conducted at Kurram Agency, Pakistan during 2007. The rhizobium inoculation significantly enhanced the growth and yield parameters of groundnut cultivars. However, the maximum plant height (88.43 cm), number of shoots (19.9 plant), number of leaves (173.27 plant), num...

Rhizobium strains from nodules of Vicia faba were diverse in plasmid content and serology. Results of multilocus gel electrophoresis and restriction fragment length polymorphism indicated several deep chromosomal lineages among the strains. Linkage disequilibrium among the chromosomal types was detected and may have reflected variation of Rhizobium strains in the different geographical location...

We present the genome sequence of Rhizobium sp. strain CCGE510, a nitrogen fixing bacterium taxonomically affiliated with the R. leguminosarum-R. etli group, isolated from wild Phaseolus albescens nodules grown in native pine forests in western Mexico. P. albescens is an endangered bean species phylogenetically related to P. vulgaris. In spite of the close host relatedness, Rhizobium sp. CCGE51...

We have developed a simple system to clone indigenous Rhizobium plasmids into E. coli. The strategy consists of three matings: the first is to insert Tn5 in the plasmid to be cloned, the second incorporates the integrative vector into the inserted Tn5 in the native Rhizobium plasmid, and the last mating transfers the target plasmid directly into E. coli. This mating-based system was successfull...