Biotransformation of isoflavones glycosides into the aglycone form is essential to attain the maximum bioavailability. The factors affecting deglycosylation of genistin in soy flour using commercial β-glucosidase enzyme were evaluated. The presence of genistin in soy flour was confirmed by isolation through chromatographic fractionation and identification by spectral method. Two-levels Plackett...

omentin-1 is a novel adipokine that is predominantly secreted by visceral adipose tissue. circulating levels of omentin-1 is inversely related to obesity. there is limited information about the effect of exercise training on serum omentin-1 concentration. the aim of this study was to investigate the effect of four weeks of progressive resistance training on serum omentin-1 levels. 16 male wiste...

results low concentrations of agnps and short incubation times can have the maximum positive impact on the peroxidase activity, and in the present study the highest activity was seen at a concentration of 40 mg/l and two hours of incubation time. conclusions this study suggests that changes of enzyme activity can occur as a result of the effect of silver nanoparticles on enzyme conformation, in...

effects of atp and mg2+ concentrations on bioluminescence reaction of luciferase (photinus pyralis) were investigated by home-made luminometer. the michaelis constants of the enzyme for atp and mg2+ obtained from the lineweaver-burk graph, were 61.9 mm ±3.3 mm and 251.6mm ± 39.0mm respectively. optimum concentration of mg2+ for maximum luminescence was 0.004 m.

Ciliate and yeast telomerase possess a nucleolytic activity capable of removing DNA from the 3' end of a single-stranded oligonucleotide substrate. The nuclease activity is thought to assist in enzyme proofreading and/or processivity. Herein, we report a previously uncharacterized human telomerase-associated nuclease activity that shares several properties with ciliate and yeast telomerases. Pa...

the effect of the fungus, metarhizium anisopliae, sub-lethal dose of imidacloprid, the aphid, myzus persicae and the three canola cultivars under microcosm conditions inside plastic cages in greenhouse (27 ± 3°c, rh 90 ± 5 %) indicated that field and semi-field concentrations of the fungus significantly reduced the population of green peach aphid (gpa) as well as in combination with lc10 of imi...

The effect of epinephrine on phosphatidate phosphohydrolase (PAP) activity of isolated human hepatocytes was studied. Epineprine inhibited the enzyme activity progressively at concentrations above 0.1 µM, reaching a maximum inhibition of 64.5% at 100 µM concentration. Inclusion of alprenolol, a β-receptor blocker, in the incubation mixture abolished the inhibitory effect of epinephrine on P...