Inorganic arsenic has strong human carcinogenic potential, but the availability of an animal model to study toxicity is extremely limited. Here, we used the transgenic zebrafish line Tg(k18(2.9):RFP) as an animal model to study arsenite toxicity. This line was chosen because the red fluorescent protein (RFP) is expressed in stratified epithelia (including skin), due to the RFP reporter driven b...

The Object Management Group (OMG) has solicited responses to a Request for Proposal (RFP) covering an Object Query Service (OQS). This OQS RFP forms part of [OMG94]. The OQS, together with others, forms part of the OMG Object Management Architecture [OMG92] for defining open, distributed object systems. This paper discusses three candidate object models that could form the basis of the OQS, nam...

We have developed a simple yet powerful technique for delineating the morphological events of tumor-induced angiogenesis and other tumor-induced host processes with dual-color fluorescence. The method clearly images implanted tumors and adjacent stroma, distinguishing unambiguously the host and tumor-specific components of the malignancy. The dual-color fluorescence imaging is effected by using...

Computational design has been used with mixed success for the design of protein surfaces, with directed evolution heretofore providing better practical solutions than explicit design. Directed evolution, however, requires a tractable high-throughput screen because the random nature of mutation does not enrich for desired traits. Here we demonstrate the successful design of the β-sheet surface o...

BACKGROUND The present study evaluated the prognostic significance of Doppler-demonstrated left ventricular (LV) restrictive filling pattern (RFP) in patients with thalassaemia major (TM), which carries an adverse cardiovascular prognosis. METHODS AND RESULTS The study group comprised 45 asymptomatic transfusion-dependent patients with TM and normal LV systolic function. All patients were che...

Reversed field pinch (RFP) discharges have been produced in a large (1.39 metre major radius, 0.56 metre average minor radius), thick walled (5 cm), aluminium vacuum vessel with indented sides. The discharges are self-reversed and ramped up to a current of 300 kA over a time of 10 ms. Reversal is sustained for £ 10 resistive diffusion times, despite the presence of large magnetic fluctuations. ...

A simple large-aspect-ratio (R0=r) circular equilibrium model is developed for low-beta reversed field pinch (RFP) geometry. The model is suitable for treating small scale instability and turbulent transport driven by ion temperature gradient (ITG) and related electron drift modes in gyrokinetic simulations. The equilibrium model is an RFP generalization of the common tokamak s-a model to small...

Rats with fluorescent markers are of great value for studies that trace lineage-specific development, particularly those assessing the differentiation potential of embryonic stem cells (ESCs). The piggyBac (PB) transposon is widely used for the efficient introduction of genetic modifications into genomes, and has already been successfully used to produce transgenic mice and rats. Here, we gener...

Drug-resistant Mycobacterium tuberculosis can be rapidly diagnosed through nucleic acid amplification techniques by analyzing the variations in the associated gene sequences. In the present study, a locked nucleic acid (LNA) probe-based real-time PCR assay was developed to identify the mutations in the rpoB gene associated with rifampin (RFP) resistance in M. tuberculosis. Six LNA probes with t...

BACKGROUND Decreases in the bioavailability of rifampicin (RFP) can lead to the development of drug resistance and treatment failure. Therefore, we investigated the relative bioavailability of RFP from one four-drug fixed-dose combination (FDC; formulation A) and three two-drug FDCs (formulations B, C, and D) used in China, compared with RFP in free combinations of these drugs (reference), in h...