Reverse transcriptase-PCR (RT-PCR) is a powerful tool for gene expression analysis at the RNA level, primarily due to its sensitivity, speed, ease of use, and versatility. In a typical RT-PCR procedure, a reverse transcriptase is used to generate a complementary DNA (cDNA) copy of RNA molecules, followed by polymerase chain reaction (PCR) to amplify the cDNA. Since the PCR process part is usual...

Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in  Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During  growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...

Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...