background and aims: canine distemper (cd) is a deadly infectious disease of canidae family. cd is a multi-systemic viral disease and is specified by wide range of clinical symptoms. the manifestations are not always indicative of cd, therefore a laboratory confirmation is necessary for suspected cases. materials and methods: different clinical specimens of 19 cd suspected unvaccinated dogs wer...

Various approaches can now be taken for amplification of RNA transcripts using the polymerase chain reaction (PCR). Here, we compare three such methods: (i) uncoupled reverse transcription (RT)-PCR (using separate reactions for cDNA synthesis and PCR), (ii) continuous RT-PCR (in which RT and DNA amplification occur in an uninterrupted reaction) using either a single enzyme for both RT and DNA a...

Several methods for the detection of Borna disease virus (BDV) RNA have been reported, one being the reverse transcription-nested polymerase chain reaction (RT-nested PCR) method. However, due to the possibility of contamination of the cloned DNA in a reaction tube, false-positive results might be obtained by RT-nested PCR. To detect only BDV RNA without anxiety of contamination, we developed a...

This review presents the basic principles of application loop-mediated isothermal amplification (LAMP) reaction for rapid diagnosis coronavirus infection caused by SARS-CoV-2. The technical details method, and most popular approaches specific non-specific detection products are briefly described. We also discuss first published works on use method nucleic acid SARS-CoV-2 virus, including those ...


 Since December 2019, COVID 19 pandemic has devastated communities across the world. As number of patients recovered from continue to rise, question acquired immunity versus chances re-infection becomes critical understand future spread infection. Here, we present a case patient previously COVID-19, develops new symptoms concerning for possible with positive reverse transcriptase-polymera...

We performed serial nested reverse transcription polymerase chain reaction (RT-PCR) assays to monitor minimal residual disease (MRD) in 25 patients with t(8;21) acute myeloid leukemia (AML). We found transient disappearance of the AML1-ETO transcript in most of the patients in complete remission. Durable PCR-negativity was found in a subgroup of patients and was associated with a good prognosis.

Vascular endothelial growth factor (VEGF)-induced angiogenesis contributes to inflammatory bone resorption in humans. Widely documented antagonists include antibiotics and non-steroidal anti-inflammatory drugs (NSAIDs). The purpose of this study was investigate the effect these on proangiogenic VEGF levels periradicular lesions. Periapical tissue biopsies were obtained from 42 patients with chr...

The citrus systemic diseases, including Huanglongbing (caused by Candidatus Liberibacter asiaticus (CLas)), tristeza virus (CTV)), tatter leaf (CTLV)), and exocortis viroid (CEVd)), are threats to production in Taiwan. Reliable diagnostic methods important for the management of these diseases. In this study, we developed a multiplex reverse transcription–polymerase chain reaction (RT-PCR) assay...

Objectives: To compare the performance of chest CT and Real-Time Reverse Transcription-Polymerase Chain Reaction (RTPCR) in initial diagnostic assessment coronavirus disease COVID-19 patients during pandemic. Methods& Results:In this study we have included 209 clinically suspected cases COVID-19from Government Medical College, Nagpur who undergone both HRCT RT-PCR. In our study, 3.35% were ...