نتایج جستجو برای: reverse dot blot
تعداد نتایج: 176482 فیلتر نتایج به سال:
Background: This study aimed to design and analyze the applicability of an oligonucleotide probe in radioisotope 32P-based dot blot hybridization for detection of hepatitis C virus. Methods: Forty-six of plasma samples were used. The plasma was extracted to obtain viral RNA genome as template for RT-PCR and the amplicon was used for nested PCR. Twenty-four HCV genomes were retrieved from GeneBa...
BACKGROUND Direct smear examination with Ziehl-Neelsen (ZN) staining for the diagnosis of pulmonary tuberculosis (PTB) is cheap and easy to use, but its low sensitivity is a major drawback, particularly in HIV seropositive patients. As such, new tools for laboratory diagnosis are urgently needed to improve the case detection rate, especially in regions with a high prevalence of TB and HIV. OB...
Diversity among the pathogenic strains of Theileria equi (T. equi), a major agent of equine piroplasmosis, can affect the appropriate detection of parasite and host immunization. Production of recombinant surface proteins from an infected horse in natural endemic area provides a reliable tool for immunodiagnosis of parasite. Regarding this, the present study was targeted toward the cloning, exp...
size of 150 (p >0.05). The cost of the consumables for the assay is approximately 10% less expensive than the dot blot assay. A plate containing 80 samples can be analyzed in under 30 min by the fluorescence-based assay, an eighth of the time required per sample for the dot blot; a single operator can easily assay over 1100 samples per day, compared to 240 samples by dot blot. Furthermore, the ...
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