An optimization strategy for ternary solvent-strength gradient elution RP chromatography is described in which a two-dimensional model of gradient time (2 levels) against ternary proportions of organic modifiers (4 levels) was constructed. From the resolution surface the optimum ratio of organic modifiers could be selected. Excellent retention time and acceptable peak width and resolution simul...

Across evolution, dolichols and polyprenols serve as sugar carriers in biosynthetic processes that include protein glycosylation and lipopolysaccharide biogenesis. Liquid chromatography coupled with electrospray ionization mass spectrometry offers a powerful tool for studying dolichols and polyprenols in their alcohol or glycan-modified forms in members of all three domains of life. In the foll...

the use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (sds) and polyoxyethylene (23) dodecanol (brij-35) as the mobile phase in reversed-phase liquid chromatography was studied. a group of chlorophenols was used as the test mixture. adding organic modifier to the system showed that the use of low concentrations of organic additives improves efficiency in sds/brij-35 mixe...