نتایج جستجو برای: restriction enzyme
تعداد نتایج: 308199 فیلتر نتایج به سال:
The restriction-modification systems use epigenetic modification to distinguish between self and nonself DNA. A modification enzyme transfers a methyl group to a base in a specific DNA sequence while its cognate restriction enzyme introduces breaks in DNA lacking this methyl group. So far, all the restriction enzymes hydrolyze phosphodiester bonds linking the monomer units of DNA. We recently r...
Restriction fragment length polymorphism (RFLP) of chromosomal DNA is one of the powerful molecular tools used in the fingerprinting of microorganism and epidemiological studies. In a wet-lab setting, pattern-based classification of organism using RFLP begins with the digestion of DNA with one to two restriction enzymes, which is followed by gel electrophoresis. This wet-lab approach may not be...
We report a case of corneal ulcer caused by nontuberculous mycobacteria which was confirmed by smear and culture. We attempted a new method for the rapid identification of mycobacteria to the species level on the basis of evaluation by the polymerase chain reaction of the gene encoding. The method is involved with restriction enzyme analysis of PCR product obtained with primers common to all my...
OBJECTIVE To construct and identify multi-gene recombinant expression vector pcDNA3-HBsAg-p30-ROP2. METHOD Primers were designed according to the gene sequences of restriction enzyme cutting site of recombinant pcDNA3-p30-ROP2 and hepatitis B surface antigen (HBsAg). The target fragment of HBsAg was amplified and cloned to expression vector pcDNA3-p30-ROP2 by restriction enzyme digestion and ...
an experiment was conducted to evaluate the effect of feed restriction (fr) and meal feeding (mf) on performance, carcass characteristics and related parameters to ascites in ross 308 male broiler chickens. five dietary treatments were 1) the control (ad libo. feeding), 2 )feed restriction from 7 to 14 days (fr14), 3) feed restriction from 7 to 21 days (fr21), 4) meal feeding from 7 to 14 days ...
Certain type II restriction modification gene systems can kill host cells when these gene systems are eliminated from the host cells. Such ability to cause postsegregational killing of host cells is the feature of bacterial addiction modules, each of which consists of toxin and antitoxin genes. With these addiction modules, the differential stability of toxin and antitoxin molecules in cells pl...
Molecular typing methods were compared by using 66 ampicillin-resistant, non-beta-lactamase-producing Enterococcus faecium clinical isolates from diverse geographic areas. Whole-plasmid analysis, restriction enzyme analysis of plasmid DNA with EcoRI and HindIII, and contour-clamped homogeneous electric field electrophoresis with digestion by SmaI and ApaI were performed on all isolates. Whole-p...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید