Restriction enzymes represent a class of endonucleases that cleave DNA in a specific manner. They are widely used in recombinant DNA technology and known as molecular scissors. In this communication, a putative restriction endonucleases from (Acinetobacter baumannii SDF) was identified in NCBI database, and characterized for biophysical properties, sequence homology and a structural modeling. T...

Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5' C A G decrease C T G 3' 3' G T C increase G A C 5' and cleave as indicated by the arrow (decrease). PvuI is an isoschizomer of XorII, RshI, and XniI. No enzyme with the specificit...