This experiment wascarried out to explore the effect of use of combinations of permeating cryoprotectant and non permeating cryoprotectant on thequality of frozen-thawed Beluga (Huso huso) spermas compared to the use of permeating cryoprotectant as a control. For this work methanol (MeOH) and dimethyl sulfoxide (DMSO)are used aspermeatingcryoprotectantsand bovine serum albuminas a non permeatin...

Results: Serum samples became PCR-positive before semen samples. During the first 6 days after inoculation, serum was PRRSV-positive in 36 of 40 boars, and semen was PRRSV-positive in four of 40 boars. Median time to detection was 36 and 48 hours for nested PCR and Taqman PCR, respectively. Results were inconsistent when a positive semen sample was pooled with negative semen. Elevated rectal te...

Bangladesh possesses a tropical goat breed popularly known as Black Bengal goat. They are dwarf in size and noted to be famous for their adaptability, fertility, prolificacy, delicacy of meat and superior skin quality (Husain et al. 1996). It is well adapted to hot and humid climate and usually produces twins and triplets. However, it has been observed that there exists sizeable genetic variati...

Neosporosis is caused by the protozoan parasite, Neospora caninum which is major cause of fatal disease and abortion in cattle in Iran and in many countries. The economic impacts of neosporosis are lost milk, meat and abortions in cattle. The purpose of the present study was to evaluate the presence of N. caninum DNA in fresh and frozen semen samples of Iranian bulls that were used for artifici...

bovine leukemia provirus: evidence of presence of part of gag gene inseminal plasma of naturally infected bullsit is of critical importance to understand the modalities of blv presence in semen, especially with regard to artificial insemination (ai). presence of bovine leukemia provirus was demonstrated in fresh and frozen semen samples by researchers. in this study paired blood and semen sampl...

this study was conducted to determine chromatin integrity and dna damage by dna electrophoresis and comet assays of buffalo fresh and frozen semen. semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and dna damage. a significant variation was found in semen parameters after thawing. highly sig...

This study was carried out to investigate effects of copper sulphate (CuSO4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and DNA damage, after semen dilution and cryopreservation. Semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. A total number of 25 samples were u...

AIM The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters. MATERIALS AND METHODS A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender whic...

This study aimed to investigate the usability of different diluents containing 6% Dimethylformamide (DMF) for cryo-preservation semen geese (Anser cygnoides). The Glucose (G), Tris-Glucose (T), Lactated Ringer’s-Glucose (LG), and Ringer’s (L), all which contained DMF, were used as cryoprotectants. researchers collected samples from four geese, twice a week over four-week period, by means abdomi...