background: distinction of hydatidiform moles (hms) from non-molar abortions and sub-classification of hms are important for clinical practice; yet, diagnosis based solely on morphology is affected by interobserver variability. the objective of this study was to determine the role of dna flow cytometry in distinguishing molar from non-molar pregnancies. materials and methods: this retrospective...

Current therapeutic strategies cannot eradicate hepatitis B virus covalently closed circular DNA (HBV cccDNA), which accounts for the persistence of HBV infection. Very recently, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‑associated protein 9 (Cas9) system has been used as an efficient and powerful tool for viral genome editing. Given that the primary duck hepa...

Abstract So-called respectively readings have posed serious challenges for theories of syntax and semantics. Sentences like George and Martha respectively denounced and were denounced by the governor (McCawley 1998) show that although the conjoined verbal expressions share the same syntactic subject, they do not predicate that subject in the same way; George (not Martha) denounced the governor,...

This study compared the potential of assessing sperm DNA fragmentation (SDF) from neat semen and the subsequent swim-up (SU) procedure to predict pregnancy when conducting ICSI of fertile donor oocytes. Infertile females (n=81) were transferred embryos resulting from intracytoplasmic sperm injection (ICSI) of their partner's spermatozoa and proven donor oocytes. This model normalized the impact...

Despite a clear link between ataxia-telangiectasia mutated (ATM)-dependent phosphorylation of p53 and cell cycle checkpoint control, the intracellular biology and subcellular localization of p53 phosphoforms during the initial sensing of DNA damage is poorly understood. Using G0-G1 confluent primary human diploid fibroblast cultures, we show that endogenous p53, phosphorylated at Ser15 (p53Ser1...

Haploid microspore-derived embryos (MDEs) of wheat were obtained by in vitro androgenesis. These  embryos were employed to evaluate the transient expression of GUS gene (uidA) following particle bombardment. Using the Bio-Rad PDS-1000/He system, the physical parameters including rupture disk pressure (900, 1100 and 1350 psi); microprojectile travel distance (6 and 9 cm); gold particles size (0....

The deep sea of temperate south-eastern Australia appears to be a ‘hotspot’ for diversity and endemism conoidean neogastropods the family Raphitomidae. Following series expeditions in region, considerable amount relevant DNA-suitable material has become available. A molecular phylogeny based on this facilitated identification diagnostic morphological characters, allowing circumscription monophy...

Protoplasts of a respiration-deficient rho strain of Saccharomyces cerevisiae were incubated with mitochondria isolated from various respiration-competent yeast species under conditions enabling transplacement of mitochondria. Respiration-competent cybrids were selected by plating the protoplasts on agar media containing a non-fermentable energy source. The resulting cybrids contained nuclear D...

The QuantifilerTM Human DNA Quantification Kit and the QuantifilerTM Y Human Male DNA Quantification Kit were designed for the quantification of human genomic DNA in forensic samples. The kits use a real-time PCR-based process to quantify, respectively, total human DNA or human male DNA only. We report the results of a developmental validation study that we performed with the Quantifiler Kits, ...

The Quantifiler Human DNA Quantification Kit and the Quantifiler Y Human Male DNA Quantification Kit were designed for the quantification of human genomic DNA in forensic samples. The kits use a real-time PCR-based process to quantify, respectively, total human DNA or human male DNA only. We report the results of a developmental validation study that we performed with the Quantifiler Kits, foll...