نتایج جستجو برای: reporter gene
تعداد نتایج: 1151125 فیلتر نتایج به سال:
Random insertions of promoterless reporter genes in genomes are a common tool for identifying marker lines with tissue-specific expression patterns. Such lines are assumed to reflect the activity of endogenous promoters and should facilitate the cloning of genes expressed in the corresponding tissues. To identify genes active in seed organs, plant DNA flanking T-DNA insertions (T-DNAs) have bee...
CRISPR/Cas9 system is a powerful gene editing tool in vivo and in vitro. Currently, CRISPR/Cas9 delivery cells or tissue with different vehicles are available, and Adeno- associated virus (AAV) in one of them. Due to AAV packaging size limitation, AAV base vectors that carry CRISPR/Cas9 system do not have florescent tag like GFP for simple detection and navigation of cells, containing AAV. The ...
Sorrel (Rumex acetosa L.) is a model dioecious plant in genetic and molecular studies for sex determination. In this research, gus reporter gene transformation to leaf disks of this plant via Agrobacterium tumefaciens was evaluated based on transient expression of this gene. Three strains of Agrobacterium( LBA4404, C58 and EHA101) and two kinds of bacterial suspensions (suspension I: Agrobacter...
Background & Objective: In order to enhance the expression of soluble proteins and facilitate their purification and development of multi-functional polypeptide , chimerical recombinant proteins have been invented . The purpose of this study was to construct ctxB-gfp-stxB gene cassette to measure the uptake and excretion of chimerical antigen in future studies. Materials & Methods: After prep...
Sorrel (Rumex acetosa L.) is a model dioecious plant in genetic and molecular studies for sex determination. In this research, gus reporter gene transformation to leaf disks of this plant via Agrobacterium tumefaciens was evaluated based on transient expression of this gene. Three strains of Agrobacterium( LBA4404, C58 and EHA101) and two kinds of bacterial suspensions (suspension I: Agrobacter...
UNLABELLED Reporter gene imaging has great potential for many clinical applications including the tracking of transplanted cells and monitoring of gene therapy. However, currently available reporter gene-reporter probe combinations have significant limitations with the biodistribution of the reporter probe and the specificity and immunogenicity of the reporter gene. The objective of the present...
gene transfer to plants and the production of transgenic plants with various purposes, such as improving the performance and quality, resistance to pests, diseases, etc., and of great importance are carried out gene transfer to plants performs to evaluate the transient and permanent gene expression. transient expression is quick, easy and simple and is not influenced by position effect compare ...
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