The present study examined the possible role of transporters in the drug-drug interactions between methotrexate (MTX) and nonsteroidal anti-inflammatory drugs (NSAIDs) in the renal uptake process of MTX. MTX is recognized by reduced folate carrier (RFC-1) and rat organic anion transporters (rOat1 and rOat3) as a substrate. Uptake of MTX by kidney slices was saturable and inhibited potently by d...

Renal disposition of administered inorganic mercury was studied in rats that had undergone an acute bilateral ureteral ligation shortly before being injected with a nontoxic 0.5-micromol/kg iv dose of inorganic mercury with or without 2.0 micromol/kg glutathione (GSH) or cysteine. Ureteral ligation and induction of "stop-flow" conditions were carried out to decrease glomerular filtration rate t...

The substrate specificity of the avian renal organic cation exchanger was examined in isolated renal brush-border membrane vesicles. Endobiotic and xenobiotic organic cations (OCs) were tested at a concentration of 100 microM for cis-inhibition of 14C-tetraethylammonium (TEA)/H+ exchange and at 1 mM for trans-stimulation of 14C-TEA efflux. The xenobiotic cations amiloride, cimetidine, mepiperph...

UNLABELLED This work addresses the issue of using (18)F-FDG PET in patients with renal failure. METHODS A model analysis has been developed to compare tissue (18)F-FDG uptake in a patient who has normal renal function with uptake in a theoretic limiting case that assumes tracer plasma decay is tracer physical decay and is trapped irreversibly. RESULTS This comparison has allowed us to propo...

1. Cyclic adenosine 3’,5’-monophosphate and N6-2’-Odibutyryl cyclic adenosine 3’,5’-monophosphate stimulate the active cellular entry and cumulative uptake of several amino acids in rat kidney cortex slices. 2. Parathyroid hormone, known to augment cellular production of cyclic adenosine 3’,5’-monophosphate by stimulating renal cortical adenyl cyclase, increases the renal cortical uptake of ami...

The aim of this study was to characterize the uptake mechanism of gentamicin, an aminoglycoside antibiotic, in human renal proximal tubular cell line HK-2. Sodium-dependent uptake of D-[(3)H]glucose and L-[(3)H]alanine was observed in HK-2 cells, indicating that the cells employed in this study retain functional characteristics of the renal proximal tubular cells. On the other hand, mRNA and pr...