ment " she gives a detailed and practical account of her system. Her prime concern is to make music itself a lived experience, by a bodily response to its many sides. Like that great pioneer and musical genius, Dalcroze, she has " discovered " that the way to teach music and all it meanj to the child, is to let him enact it with his own body. So too, like Dalcroze, she has found that lived musi...

Claude Shannon was born on April 30, 1916 in the town of Gaylord, Michigan. As Gallager describes it, he “led a normal happy childhood with little indication of his budding genius” [1]. Friendly, but not-outgoing, he showed a curiosity for how things worked. He attended public high school and graduated when he was sixteen, and earned his Bachelor’s degrees in electrical engineering and mathemat...

Felix Mendelssohn, Goethe's favorite, who revealed the genius ofJohann Sebastian Bach after almost a century of oblivion, was of a fragile constitution. Last year of his life he was very depressed because of his sister Fanny's sudden death. The last month he had several attacks of severe headaches with agitation, anxiety, confusion and slow pulse. Last three days these episodes became more freq...

Bending of purple membranes in dependence on the pH analyzed by AFM and single molecule force spectroscopy R.-P. Baumann, M. Schranz and N. Hampp, Phys. Chem. Chem. Phys., 2010 DOI: 10.1039/b919729j Bifunctional polyacrylamide based polymers for the specific binding of hexahistidine tagged proteins on gold surfaces Lucas B. Thompson, Nathan H. Mack and Ralph G. Nuzzo, Phys. Chem. Chem. Phys., 2...

We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

NIVERSITIES are hostile to Genius," said ' Emerson. He might have cited the University SI of Paris and Vesalius as a case in point. For here was a genius who had articled with destiny to dedicate himself, his gifts, his splendid impatience to Reform; and here a University so encrusted and anklyosed with Conservatism that there was nothing quite like it in Europe at the time. What was Vesalius d...

Bacteria perform astounding feats inside host cells, demonstrating that they are able to exploit principles of cell biology that so far have eluded some of the brightest human minds. Included in their repertoire is the ability to recruit cytoskeletal elements, appropriating them to the task of bacterial locomotion. Several species of three unrelated bacterial genera — Listeria, Shigella and Ric...