Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybrid approach simplifies the experiment and increases the data acquisition speed. It also removes th...

Introduction Conclusions References

Computerized classification of surface spikes in three-dimensional electron microscopic reconstructions of viruses

Climatic variations at decadal scales such as phases of accelerated warming or weak monsoons have profound effects on society and economy. Studying these variations requires insights from the past. However, most current reconstructions provide either time series or fields of regional surface climate, which limit our understanding of the underlying dynamics. Here, we present the first monthly pa...

Background Pre-surgical planning for Fontan procedures involves time-consuming patient-specific vascular segmentation and non-intuitive virtual surgical modeling which has encumbered utility in the clinic by surgeons. We present an interactive platform to design virtual surgical options and visualize pericardial enlargement strategy for Fontan connections, starting with patient-specific vascula...

Many practical systems can be described by dynamic networks, for which modern technique can measure their outputs, and accumulate extremely rich data. Nevertheless, the network structures producing these data are often deeply hidden in the data. The problem of inferring network structures by analyzing the available data, turns to be of great significance. On one hand, networks are often driven ...