background: oncolytic herpes simplex virus (ohsv) vectors lacking γ34.5 has demonstrated anticancer effects without affecting normal cells. therefore, they are considered as ideal templates to construct efficient vectors for tumor targeting and cancer gene therapy. herein, we reported construction of three single/dually-labeled recombinant hsv-1 vectors, which provide an opportunity to replace ...

objective(s) lipocalin 2 (lcn2) is a 25-kda glycoprotein that has initially been extracted from neutrophil granules. expression of lcn2 is induced under various pathophysiological conditions. it is also known as an early marker of kidney and heart injury. high-level expression of recombinant lcn2 neutrophil gelatinase-associated(ngal) in insect cells was the aim of this study. materials and met...

Chromosomal rearrangements can result from crossing over during ectopic homologous recombination between dispersed repetitive DNA. We have previously shown that meiotic ectopic recombination between artificially dispersed ade6 heteroalleles in the fission yeast Schizosaccharomyces pombe frequently results in chromosomal rearrangements. The same recombination substrates have been studied in mito...

1. General Recombination 2. Recombination and Pairing of Homologous Chromosomes 3. Induction of Recombination 4. Establishment of Recombination 5. Structure and Function of Synaptonema Complex. 6. Transcription and Recombination 7. Somatic recombination/ Diploid Genetics 8. Genes and Proteins in Meiotic Cells / Haploid Cell Genetics 9. Meiosis and Environment 10. Conclusion Glossary Bibliograph...

As a key evolutionary process, recombination shapes the genetic structure of virus populations. The increased availability of virus sequences provides a chance to study virus recombination through molecular data. Many statistical methods have been developed, and a lot of the methods are phylogenetic-based. My research focuses on recombination modeling and data analysis. I first apply an existin...

We describe a genetic system for studying the reciprocality of chromosomal recombination; all substrates and recombination functions involved are provided exclusively by the bacterial chromosome. The genetic system allows the recovery of both recombinant products from a single recombination event. The system was used to demonstrate the full reciprocality of three different types of recombinatio...

Meiotic recombination is an important driver of evolution. Variability in the intensity of recombination across chromosomes can affect sequence composition, nucleotide variation and rates of adaptation. In many organisms recombination events are concentrated within short segments termed recombination hotspots. The variation in recombination rate and recombination hotspot positions can be studie...

Recombination can be a dominant force in shaping genomes and associated phenotypes. To better understand the impact of recombination on genomic evolution, we need to be able to identify recombination in aligned sequences. We review bioinformatic approaches for detecting recombination and measuring recombination rates. We also examine the impact of recombination on the reconstruction of evolutio...