Serum samples obtained from white-tailed deer (Odocoileus virginianus) in Connecticut (n=218) and South Carolina (n=20) (USA) during the period 1992-2002 were analyzed for antibodies to whole-cell or recombinant antigens (i.e., fusion proteins) of Borrelia burgdorferi sensu stricto and Anaplasma phagocytophilum, etiologic agents of Lyme borreliosis and granulocytic ehrlichiosis, respectively. I...

Assessment of humoral immunity to SARS-CoV-2 and other infectious agents is typically restricted detecting antigen-specific antibodies in the serum. Rarely does immune monitoring entail assessment memory B-cell compartment itself, although it these cells that engage secondary antibody responses capable mediating protection when pre-existing fail prevent re-infection. There are few techniques ra...

Mannheimia haemolytica is responsible for considerable economic losses to cattle, sheep, and goat industries in many parts of the world. This bacterium isone of the causative agents of shipping fever in cattle. Current vaccines against M. haemolytica are moderately efficacious since they do not provide complete protection against the disease. Production of a...

Japanese encephalitis virus (JEV) is an enveloped icosahedral capsid with a prime neutralizing epitope present in E protein domain III (EDIII). dimers are rearranged into five-fold symmetry of icosahedrons. Cholera toxin B (CTB) and heat-labile enterotoxin (LTB) AB5-type was used as the structural scaffold for emulating pentameric axis EDIII. We produced homo-pentameric EDIII through genetic fu...

The molecular tag vaccine against Brucella abortus and serological testing are the main methods of prevention of brucellosis used currently. They can discriminate vaccinated animals and humans from those naturally infected. In this study, we constructed a gene deletion mutant strain, B. abortus S19 virB5 with a molecular tag. Recombinant VirB5 was expressed and purified for evaluation as a diag...

A competitive-inhibition enzyme-linked immunosorbent assay (cELISA) was developed for detection of equine antibodies specific for Babesia caballi. The assay used recombinant B. caballi rhoptry-associated protein 1 (RAP-1) and monoclonal antibody (MAb) 79/17.18.5, which is reactive with a peptide epitope of a native 60-kDa B. caballi antigen. The gene encoding the recombinant antigen was sequenc...

The gene coding for the major outer membrane protein Omp31 was sequenced in five Brucella species and their biovars. Although the omp31 genes appeared to be highly conserved in the genus Brucella, nine nucleotide substitutions were detected in the gene of Brucella ovis compared to that of Brucella melitensis. As shown by differential binding properties of monoclonal antibodies (MAbs) to the two...

results the results showed that sensitivity and specificity were respectively 84.12% and 92% for‎ recombinant protein srs3 and 92% and 96% for rsag1. conclusions recombinant proteins srs3 and rsag1 are promising antigens in diagnostic assays for detection of specific antibodies against t. gondii. background infections by the protozoan parasite toxoplasma gondii (t. gondii) are widely prevalent ...

objective(s): production of a recombinant and immunogenic antigen using dengue virus type-3 envelope protein is a key point in dengue vaccine development and diagnostic researches. the goals of this study were providing a recombinant protein from dengue virus type-3 envelope protein and evaluation of its immunogenicity in mice. materials and methods: multiple amino acid sequences of different i...

in the last decades researchers had focused on developing a vaccine against tick based on protective antigen. recombinant vaccines based on concealed antigen from boophilus microplus have been developed in australia and cuba by the name of tickgard and gavac (de la fuente and kocan, 2006). further studies on this antigen have shown some extent of protection against other species (de vos et al.,...