SARS-CoV-2 nucleocapsid (N) protein has been proposed as a good vaccine target. N-specific T cells were observed in N immunized mice and COVID-19 convalescents. It is of importance to identify the cell responses triggered by protein. Intradermal immunization with SARS-CoV was demonstrated elicit non-protective which may be avoided intranasal vaccination. Therefore, we conducted vaccination BALB...

African Swine Fever Virus (ASFV) is a high-consequence transboundary animal pathogen that often causes hemorrhagic disease in swine with a case fatality rate close to 100%. Lack of treatment or vaccine for the disease makes it imperative that safe and efficacious vaccines are developed to safeguard the swine industry. In this study, we evaluated the immunogenicity of seven adenovirus-vectored n...

To evaluate the possible effects of LMP2A (EBV latent membrane protein 2A) on human gastric cancer cell line SGC-7901, LMP2A coding gene was subcloned into shuttle plasmid pAdTrackCMV to form transfer plasmid pAdTrackCMV-2A, which was linearized with PmeI and cotransformed into E.coli BJ5183 with adenovirus genomic plasmid of pAdeasy-1. The identified recombinant adenovirus plasmid DNA was dige...

The inability of adenovirus to infect primitive hematopoietic cells presents an obstacle to the use of adenovirus vectors for gene transfer to these cell types. Therefore, expanding the tropism of adenovirus vectors to unique cell surface antigens would be an important development for gene therapy protocols. In this study, we sought to redirect infection of adenovirus vectors to primitive human...

We previously reported that systemic injection of recombinant adenovirus resulted in a rim of gene transduction around experimental liver tumor nodules. This zone of higher infection is dependent on the avb3 integrin, acting as an adenovirus internalization receptor, which is overexpressed in tissues surrounding liver metastases. When a recombinant adenovirus encoding interleukin-12 (AdCMVIL-12...

Adenoviruses are potent vectors for inducing and boosting cellular immunity to encoded recombinant antigens. However, the widespread seroprevalence of neutralizing antibodies to common human adenovirus serotypes limits their use. Simian adenoviruses do not suffer from the same drawbacks. We have constructed a replication-deficient chimpanzee adenovirus-vectored vaccine expressing the conserved ...

This project aimed to test a new vaccine technology platform using an adenovirus vector to produce a recombinant vaccine against severe acute respiratory syndrome (SARS) infection. A recombinant adenovirus (rAd) encoding SARS coronavirus (SARS-CoV) nucleocapsid protein (rAd-N) was constructed, and the ability of the rAd-N to induce anti–SARS-CoV N antibody production and cellular immune respons...

Abstract Background ChAdOx1 nCoV-19 is a recombinant adenovirus vaccine against SARS-CoV-2 that has passed phase III clinical trials and now in use across the globe. Although replication-defective normal cells, 28 kbp of genes delivered to cell nucleus alongside S glycoprotein gene. Methods We used direct RNA sequencing analyse transcript expression from genome human MRC-5 A549 lines are non-pe...

objective(s):diabetic nephropathy is an important long-term complication of diabetes mellitus which appears to be partially mediated by an increase in secretion of transforming growth factor-β (tgf-β). fibromodulin, the small leucine-rich proteoglycan, has been proposed to be the potent tgfβ1 modulator. in this study, the therapeutic effects of recombinant adenoviral vectors expressing fibromod...

The susceptibility of adenovirus to the inhibitory effect of human interferons in vitro was investigated. We tested recombinant human interferons-c~2, -/~1 and -7 against adenovirus serotypes 1 and 5 (group C), 3 and 7a (group B), and a wild strain isolated from an acutely ill child who later died. Pretreatment of WISH cells with interferon-? for 24 h induced a dose-dependent inhibition of mult...