PurposeCurrent polymerase chain reaction (PCR) methods for the diagnosis of infections are time consuming and require large sample volume skilled technicians. We developed a novel, easy-to-use, rapid (processing time, 1 minute; total 33 minutes) multiplex real-time PCR test (Direct Strip PCR) that did not DNA extraction to detect 9 pathogens could cause uveitis in 20-μl samples.DesignMulticente...
