Recent evidence proposes that cells with the properties of mesenchymal stem cells (MSCs) can be extracted from adult peripheral tissues, including muscle, dermis and adipose tissue. Adipose tissue is ubiquitous and easily obtainable in large quantities. We separated Adipose derived stem cells (ADSCs) from part of subcutaneous adipose tissue from rat. These cells could be readily differentiated ...

Intraperitoneal transplantation of hepatocyte microbeads is an attractive option for the management of acute liver failure. Encapsulation of hepatocytes in alginate microbeads supports their function and prevents immune attack of the cells. Establishment of banked cryopreserved hepatocyte microbeads is important for emergency use. The aim of this study was to develop an optimised protocol for c...

Gupta, Sanjeev, Pankaj Rajvanshi, Emma Aragona, Chang-Don Lee, Purnachandra R. Yerneni, and Robert D. Burk. Transplanted hepatocytes proliferate differently after CCl4 treatment and hepatocyte growth factor infusion. Am. J. Physiol. 276 (Gastrointest. Liver Physiol. 39): G629– G638, 1999.—To understand regulation of transplanted hepatocyte proliferation in the normal liver, we used genetically ...

Developing in vitro engineered hepatic tissues that exhibit stable phenotype is a major challenge in the field of hepatic tissue engineering. However, the rapid dedifferentiation of hepatic parenchymal (hepatocytes) and non-parenchymal (liver sinusoidal endothelial, LSEC) cell types when removed from their natural environment in vivo remains a major obstacle. The primary goal of this study was ...

BACKGROUND & AIMS Primary hepatocytes are of great importance for basic research as well as cell transplantation. However, their stability, especially in suspension, is very low. This feature severely compromises storage and shipment. Based on previous studies with adherent cells, we here assessed cold storage injury in rat hepatocyte suspensions and aimed to find a cold storage solution that p...

Hepatocytes self-assemble in culture to form compacted spherical aggregates, or spheroids, that mimic the structure of the liver by forming tight junctions and bile canalicular channels. Hepatocyte spheroids thus resemble the liver to a great extent. However, liver tissue contains other cell types and has bile ducts and sinusoids formed by endothelial cells. Reproducing 3-D co-culture in vitro ...