The effect of expression of the c-l la-ra.s oncogene on cisplatin (DDP) sensitivity was examined in murine NIH 3T3 cells transfected with the dexamethasone (DEX)-inducible mouse mammary tumor virus promoter linked to an activated c-Ha-rai gene [LTR H-ras(A) cells]. Treatment of these cells with 5 JJM DEX for 24 h induced c-Ha-ras expression and produced an 8.2 ±1.3-fold (SD) increase in DDP re...

The small guanine nucleotide-binding protein Ras, activated by peptide mitogens and other stimuli, regulates downstream signaling events to influence transcription. The role of Ras in solute signaling to gene regulation was investigated in the murine inner medullary collecting duct (mIMCD3) cell line. Urea treatment (100-200 mM), but not sham treatment, increased Ras activation 124% at 2 min; t...

We recently identified a prenyl peptide-binding protein in microsomal membranes from bovine brain (Thissen, J. A., and Casey, P. J. (1993) J. Biol. Chem. 268, 13780-13783). Through a variety of approaches, this binding protein has been identified as the cytoskeletal protein tubulin. Prenyl peptides bind to purified tubulin with a Kd of 40 nM and also bind to tubulin polymerized into microtubule...

one of the distinguishing features of implementation research is the importance given to involve implementers in all aspects of research, and as users of research. we report on a recent implementation research effort in india, in which researchers worked together with program implementers from one of the longest serving government funded insurance schemes in india, the rajiv aarogyasri scheme (...

Article history: Received: 25 Feb 2012 Accepted: 12 May 2012 Available online: 7 Jan 2013 ZJRMS 2014; 16(1): 36-39 Background: Kidney transplantation is the best treatment option for kidney failure. Major medical progress has been made in the field of renal transplantation over the last 40 years. The surgical procedure has been standardized and the complication rate is low. Overall, the outcome...

Background: K-ras oncogene mutations are confirmed factor for lack of clinical benefit from antibodies targeting EGFR in patients with colorectal cancer (CRC). Mutations are reported in 40% of CRC tumors in western patients. There is scarcity of data on population from Asia and the Middle East. Aims: This study investigates the frequency and impact of k-ras mutation and the association between ...

We tested the antineoplastic effect of the farnesyltransferase inhibitor L-744,832 in mammary and lymphoid tumors overexpressing the N-ras proto-oncogene in transgenic mice. Mice bearing mammary tumors were randomly assigned to receive daily 40 mg/kg s.c. injections of this compound (experimental group, n = 6) or vehicle (control group, n = 6) per day for 5.5 weeks. Treatment with the compound ...

The large tumor antigen of simian virus 40 (SVLT) is a potent oncogene. Although inactivation of the p53 and pRb tumor suppressors has been causally linked to the transforming properties of SVLT, its exact mechanism of action remains undefined. Previous data indicated that Ras is activated in SVLT-expressing cells. In this report we show that SVLT also increases Raf kinase activity in both inse...

Palonosetron (Aloxi(®), Onicit(®), Paloxi(®)) is a second-generation 5-HT(3) receptor antagonist (RA) with an extended half-life of ~40 hours and high binding affinity for the 5-HT₃ receptor that is markedly different from other 5-HT(3) RAs. Phase III trials demonstrate that a single dose of palonosetron compared with traditional 5-HT₃ RAs is more effective in preventing chemotherapy-induced na...