Alpha toxin derived from Clostridium perfringens was used for diagnosis of Streptococcus agalactiae (St.agalactiae) by rapid and spot CAMP tests. The selected Cl.perfringens strain was cultured in optimized conditions; the supernatant of culture was concentrated by ultra filter and purified by DEAE cellulose chromatography. The efficacy of both purified and crude α-toxins were examined by rapid...

In this study, a simple and reliable method by gas chromatograph–mass spectrometry (GC–MS) was developed for the fast and regular identification of 3, 4-MDMA impurities in ecstasy tablets. In so doing, 8 samples of impurities were extracted by diethyl ether under alkaline condition and then analyzed by GC–MS. The results revealed high MDMA levels ranging from 37.6% to 57.7%. The GC-MS method sh...

In this study, a simple and reliable method by gas chromatograph–mass spectrometry (GC–MS) was developed for the fast and regular identification of 3, 4-MDMA impurities in ecstasy tablets. In so doing, 8 samples of impurities were extracted by diethyl ether under alkaline condition and then analyzed by GC–MS. The results revealed high MDMA levels ranging from 37.6% to 57.7%. The GC-MS method sh...

abstract background: the disk diffusion method for identification of yeasts species was performed based on different but distinct susceptibilities of yeasts spp. to chemicals: janus green, ethidium bromide, 2,3,5-triphenyltetrazolium chloride, brilliant green, cycloheximide and rhodamine 6g. methods: atotal of 568 iranian soldiers went under study for isolation and identification of yeast speci...

background: dermatophytes are the most common causative agents of superficial mycoses. species identification of these fungi is important from therapeutic and epidemiological point of wive. traditional approaches for identification of dermatophytes at the species level, relying on macroscopic and microscopic features of the colonies, usually are time-consuming and unreliable in many circumstanc...

objective(s) to compare two identification methods, i.e., restriction fragment length polymorphism (rflp)-pcr analysis and enzymatic method rapid tm yeast plus system to identify different species causing vulvovaginal candidiasis (vvc). materials and methods vaginal discharges of women who had attended the gynecology outpatient clinic of mobini hospital in sabzevar, iran were collected using co...