Three monoclonal antibodies were developed to the placenta-specific glycoprotein SP1. The antibodies were used in the characterization of SP1 in placental tissue, pregnancy serum and urine, cancer serum, and cell culture. The three antibodies reacted similarly with purified SP1 of placental origin in radio- and enzyme immunoassay, and all three decorated the syncytiotrophoblast layer of placent...

this study was done on 4 and 5 years old great sturgeon cultured in brackish water ponds in bafgh salt water fisheries research station during one year. we examined somatic growth indices and effects of different diets on blood parameters and total length and weight. diet with constant protein (%40) and different energy levels (400,425,450,475 cal/100g) were fed to 74 pieces of great sturgeons ...

no report has been published on the economical losses associated with errors in oestrus detection in cows under iranian intensive dairy management. the following study was carried out to determine the accuracy of oestrus detection by farmers using artificial insemination in shiraz dairy farms. milk samples were collected from 179 holstein milking cows at the time of artificial insemination. mil...

background: since the introduction of the first radioimmunoassay, several improvements have been made in the design of immunoassays such as method of antibody production, labeling, automation and detection technology. we performed an analytical evaluation of the new electrochemiluminescent immunoassay (eclia) for serum tsh, ft4 and t3 in the elecsys 2010 immunoassay system and compared the resu...

background: aflatoxin b1 (afb1) is a toxic metaboliteproduced by aspergillus species that contaminates a wide range ofagricultural products. objectives: this study was designed todevelop a rapid and highly sensitive immunoassay method inmicroarray format for quantitative detection of afb1 to evaluatethe potential of microarray platform for high-throughput screening,which can be beneficial in fo...

Some analytical functions were tested for goodness of fit to standard curves in radio-immunoassays of insulin (Hales & Randle, 1963). Letting a, b and c be parameters, and y the radioactivity measured at an insulin concentration of x, excellent fit was obtained with the function 1/(y+c)=ax+b. This function was associated with a smaller variance around y and a greater number of sign changes for ...