To investigate the role of progesterone (P4) as a survival factor in quail granulosa cell explants, P4 content was determined under various conditions and correlated with apoptotic indexes (AIs) evaluated by 2',6'-diamidino-2-phenylindole (DAPI)-staining. Analysis of serum-free cultures from 24 to 96 h shows decreased P4 levels in the medium paralleled by increasing AI. Inhibiting apoptosis by ...

A maternidade (entendida aqui como a experiência de se tornar mãe) é, para muitas mulheres, um dos aspectos mais intensos e significativos da existência humana. Estando geralmente associada a um momento de renovação da vida, algumas vezes, a maternidade se associa a riscos para a vida da mulher ou da criança. Os riscos para a mulher podem ser maiores ou menores dependendo do grau de suporte ou ...

Oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. Progesterone (P4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...

Ins(1,3,4,5)P4 was able to mobilize the entire Ins(1,4,5)P3-sensitive intracellular Ca2+ store in saponin-permeabilized SH-SY5Y human neuroblastoma cells in a concentration-dependent manner, yielding an EC50 value of 2.05 +/- 0.45 microM, compared with 0.14 +/- 0.03 microM for Ins(1,4,5)P3. However, L-Ins(1,3,4,5)P4 [= D-Ins(1,3,5,6)P4] failed to cause mobilization of intracellular Ca2+ at conc...

We adopt the following rules: p1, p2, p3, p4, p5, p6, p, p7 denote points of E2 T and a, b, c, r, s denote real numbers. Next we state four propositions: (1) If sin](p1, p2, p3) = sin](p4, p5, p6) and cos](p1, p2, p3) = cos](p4, p5, p6), then ](p1, p2, p3) = ](p4, p5, p6). (2) sin](p1, p2, p3) = −sin](p3, p2, p1). (3) cos](p1, p2, p3) = cos](p3, p2, p1). (4) ](p1, p4, p2)+](p2, p4, p3) = ](p1, ...

The objective of this experiment was to determine whether post-mating progesterone (P4) supplement improves pregnancy and lambing rates in ewes bred during the seasonal anestrous period. In June, 39 synchronized to estrus Awassi ewes were allowed with four harnessed fertile rams immediately following CIDR-G device removal (day 0 and 0 hour). Five days following ram introduction, ewes were rando...

The article of Marc Jamoulle shows the importance of the contribution of general practitioners (GPs) in improving the quality and the efficiency of the health systems. Starting from the concept of quaternary prevention for reducing excessive costs in the preventive procedures, he suggests a change of paradigm in every daily activity of the GP in order to have a stronger ethical approach to the ...

BACKGROUND In higher primates, although LH/CG play a critical role in the control of corpus luteum (CL) function, the direct effects of progesterone (P4) in the maintenance of CL structure and function are unclear. Several experiments were conducted in the bonnet monkey to examine direct effects of P4 on gene expression changes in the CL, during induced luteolysis and the late luteal phase of n...

The effect of progesterone (P4) on fructose rich diet (FRD) intake-induced metabolic, endocrine and parametrial adipose tissue (PMAT) dysfunctions was studied in the adult female rat. Sixty day-old rats were i.m. treated with oil alone (control, CT) or containing P4 (12 mg/kg). Rats ate Purina chow-diet ad libitum throughout the entire experiment and, between 100 and 120 days of age drank ad li...

Protein kinase B (PKB/Akt) plays a key role in cell signaling. The PH domain of PKB binds phosphatidylinositol 3,4,5-trisphosphate translocating PKB to the plasma membrane for activation by 3-phosphoinositide-dependent protein kinase 1. The crystal structure of the headgroup inositol 1,3,4,5-tetrakisphosphate Ins(1,3,4,5)P4-PKB complex facilitates in silico ligand design. The novel achiral anal...