For real-time monitoring of PCR amplification of DNA, quantitative PCR (qPCR) assays use various fluorescent reporters. DNA binding molecules and hybridization reporters (primers and probes) only fluoresce when bound to DNA and result in the non-cumulative increase in observed fluorescence. Hydrolysis reporters (TaqMan probes and QZyme primers) become fluorescent during DNA elongation and the r...

Protozoan contamination in produce is of growing importance due to their capacity cause illnesses consumers fresh leafy greens. Viability assays are essential accurately estimate health risk caused by viable parasites that contaminate food. We evaluated the efficacy reverse transcription quantitative PCR (RT-qPCR), propidium monoazide coupled with (q)PCR, and viability staining using iodide thr...

Fungi constitute an important group in soil biological diversity and functioning. However, characterization and knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative PCR (real-time Q-PCR). The aim in this study was to quantify fungal abundance in soils by incorporating, into a real-time Q-PCR using the SYBRGreen®...

BACKGROUND We performed quantitative real-time polymerase chain reaction (Q-PCR) to monitor the evolution of Brucella melitensis DNA load from initial diagnosis through post-therapy follow-up in patients with brucellosis. METHODS On the basis of real-time fluorometric quantification of PCR products, we used the ultra-rapid LightCycler system (Roche Diagnostics). We collected 180 peripheral bl...

Background‘ACROSIS COVID-19 A g (NPS)’ kit (SG Medical, Seoul, Korea) is a newly developed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen-detection rapid diagnostic test (Ag-RDT) using surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFIA). We evaluated its clinical performance compared with STANDARD Q (SD Biosensor, Suwon, Korea), previously approv...

background: q fever is an important widespread reemerging zoonosis. the presence of coxiella burnetii in 100 tick-infested dogs was assessed in this study. methods: the blood samples from 100 referred dogs were acquired and evaluated by nested-pcr. results: c. burnetii was detected in 11 out of 100 (11%) blood samples. most of the positive dogs were kept outdoor and fed on raw diet. based on ou...

background: minimal residual disease (mrd) tests provide early identification of hematologic relapse and timely management of acute myeloid leukemia (aml) patients. approximately, 50% of aml patients do not have clonal chromosomal aberrations and categorize as a cytogenetically normal acute myeloid leukemia (cn-aml). about 60% of adult cn-aml has a mutation in exon 12 of npm1 gene. this mutatio...

BACKGROUND: Q fever is a zoonotic disease caused byCoxiella burnetii, a species of bacteria that is distributedworldwide. In cattle, Coxiella burnetii infections are generallyasymptomatic but can also be associated with reproductivedisorders. OBJECTIVES: The aim of this study was to achievemolecular detection of Coxiella burnetii in dairy bovine milkfarms using Nested PCR in Qom province, Iran....

Bacteriological culturing has traditionally been used to detect microbial pathogens in food, and this technique is still in widespread use today. However, the time-consuming and low-throughput nature of culture-based detection methods warrant their replacement by more rapid methods based on biomolecular analysis, like the polymerase chain reaction (PCR). PCR, predicated on DNA synthesis and amp...