There currently is more and more interest in the use of natural products, such as tea polyphenols, as therapeutic agents. The polyphenol compound pyrogallol has been reported before to inhibit quorum-sensing-regulated bioluminescence in Vibrio harveyi. Here, we report that the addition of 10 mg · liter(-1) pyrogallol protects both brine shrimp (Artemia franciscana) and giant river prawn (Macrob...

An alkaline saponification method containing pyrogallol is usually applied for the determination of tocopherols. Although there are some metabolites of ƒ¿-tocopherol in tissues, it is not yet clear whether they are true metabolites or degradation products from the alkaline saponi fication. Since it was shown that ƒ¿-tocopheryl spirodimer, a metabolite of ƒ¿-tocopherol, was degraded mainly to a ...

BACKGROUND Flowers of Bauhinia kockiana were investigated for their anticancer properties. METHODS Gallic acid (1), and methyl gallate (2), were isolated via bioassay-directed isolation, and they exhibited anticancer properties towards several cancer cell lines, examined using MTT cell viability assay. Pyrogallol (3) was examined against the same cancer cell lines to deduce the bioactive func...

A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional protocols.

this study, the effects of a number commonly used pesticides on activity polyphenol oxidase (PPO) enzyme from pesticide-free and pesticide-applied 'Jonagold' apples were comparatively evaluated. Substrates 4-methylcatechol, pyrocatechol, pyrogallol L-tyrosine to determine substrate specificity PPO obtained apple. According results, contrary, apple did not show any against which is monophenolic ...

OBJECTIVES Most clinical laboratories replaced their manual precipitation techniques for the determination of urinary protein with automated dye binding assays or benzethonium chloride-turbidimetric assays. Few studies have validated these assays for the measurement of urinary proteins in the normal range. DESIGN AND METHODS This study compares four automated assays for the measurement of uri...

Pine, grape and witch hazel byproducts are rich sources of polyphenols. From extracts (OW) soluble in both ethyl acetate and water we have generated a collection of fractions differing in percentage of pyrogallol groups. The mixtures were highly active as free radical scavengers against DPPH. The phenolics protected red blood cells from free radical induced hemolysis and were mildly cytotoxic t...

Electrogenerated chemiluminescence (ECL) of CdSe quantum dots (QDs) dispersed in aqueous solution was studied with bare electrode. The ECL emission was observed at -1.4 V vs. Ag/AgCl and the ECL spectrum peak is similar to that of the defect photoluminescence (PL) spectrum, indicating the surface defects played a critical role in the emission process. The experiment results suggested that disso...

Discs of network polyvinyl alcohol-glutaraldehyde (PVAG) were synthesized and coated with polyaniline (PANI) using glutaraldehyde as a chemical arm (PVAG-PANIG-HRP disc). The best conditions for the immobilization were established as about 1.0 mg mL(-1) of protein, for 60 min and pH 5.5. The soluble enzyme lost all of its activity after incubation at 70°C for 15 min, whereas the PVAG-PANIG-HRP ...