Purine nucleotides are critically important for the normal functioning of cells due to their myriad of activities. It is important for cells to maintain a balance in the pool sizes of the adenine-containing and guanine-containing nucleotides, which occurs by a combination of de novo synthesis and salvage pathways that interconvert the purine nucleotides. This review describes the mechanism for ...

It has been reported that the capacity of rabbit bone marrow to carry out the synthesis de novo of purine ribonucleotides is quite low, and that this tissue requires preformed purine derivatives supplied by the liver (1, 2). Presumably, the salvage enzymes, including nucleoside phosphorylases (3),. participate in the reutilization of the preformed derivatives. So far, only pyrimidine nucleoside...

Feedback inhibition of purine biosynthesis de novo in bacteria has been studied in some detail by Gots and his colleagues (l-4). Other observations have suggested feedback inhibition, but these are complicated by dilution of intermediary pools by the added inhibitor (5-10). This reaction has been studied less extensively in mammalian cells. Sartorelli and LePage (11) and LePage and Jones (12) h...

Feedback inhibition of purine biosynthesis de novo in bacteria has been studied in some detail by Gots and his colleagues (l-4). Other observations have suggested feedback inhibition, but these are complicated by dilution of intermediary pools by the added inhibitor (5-10). This reaction has been studied less extensively in mammalian cells. Sartorelli and LePage (11) and LePage and Jones (12) h...

Purine biosynthesis by the 'de novo' pathway was demonstrated in isolated rat extensor digitorum longus muscle with [1-14C]glycine, [3-14C]serine and sodium [14C]formate as nucleotide precursors. Evidence is presented which suggests that the source of glycine and serine for purine biosynthesis is extracellular rather than intracellular. The relative incorporation rates of the three precursors w...

haloalkyl purines i.e. 2,6-diamino-8-(chloromethyl) purine 10; 2, 6-diamino-x- (chloromethyl) purine 17; 2,6-diamino-8-(chloropropylp) urine 18; were synthesized by two distinct but facile methods. the n-alkylating potential of 2, 6-diamino-8- (chloromethyl) purine was investigated. the following products 2,6-diamino-8-(nbenzyl- n-methyl) purine 11; 2,6-diamino-8-(4-nitro anilinomethyl) purine ...

Eukaryotic cells acquire purines via de novo synthesis or salvage mechanisms. Unlike human cells, protozoan parasites from the Apicomplexan and Trypanosomatid lineages such as Plasmodium, Toxoplasma, Leishmania, Trypanosoma and Crithidia species lack de novo purine synthesis enzymes, and therefore rely on salvage of preformed purine bases for growth and proliferation, making purine salvage an a...

Azaserine has been shown to be an inhibitor of de novo purine synthesis (4, 8). In studies with mice bearing ascites cell tumors, inhibition of purine synthesis was shown to correlate with in creased survival time of the hosts (7). It was fur ther demonstrated that the significant effect of the drug on the tumor cells involved a reaction between azaserine and a constituent of the cells (3, 7). ...

The importance of de novo purine synthesis as opposed to the reutilisation of metabolites by salvage pathways, and the nature of the excretory product(s) of purine degradation, have been examined in cultured preimplantation mouse embryos. In the presence of azaserine and mycophenolic acid, which inhibit de novo purine synthesis, embryo cleavage was blocked prior to compaction, the precise stage...