The performance of the Capilia TB-Neo assay, a new-generation assay, was assessed by determining its sensitivity, specificity, reproducibility, and cross-reaction with contaminating organisms. The sensitivity and specificity were 99.2 and 96.4% and 89.3 and 100% in pure and mixed-culture isolates, respectively. The kappa statistic was 95.0 and 77.9% in pure and mixed culture isolates, respectiv...

A 45-year-old man with a cholesteatoma experienced purulent meningitis. Microbial analysis of cerebrospinal fluid yielded in pure culture a gram-negative bacillus. Phenotypic methods were suggestive of a Bacteroides distasonis or either a Bacteroides thetaiotaomicron or Bacteroides ovatus infection. The isolate was identified by 16S rRNA gene sequence analysis as B. thetaiotaomicron. This is th...

Strain PA-1 (S. Barik, W.J. Brulla, and M.P. Bryant, Appl. Environ. Microbiol. 50:304-310, 1985) is an anaerobic, gram-negative rod that in pure culture decarboxylates succinate to propionate and that grows syntrophically as an acetogen with the H2 utilizer Methanospirillum hungatei if glucose, pyruvate, aspartate, or fumarate is provided. In pure culture, strain PA-1 grows optimally in a mediu...

During the last 100 years, the neuston bacterium Nevskia ramosa has been described several times. This bacterium forms conspicuous rosette-like microcolonies at the air-water interface. In this study, pure cultures of Nevskia ramosa were obtained for the first time, from a bog lake (strain Soe1, DSMZ 11499T) and a freshwater ditch (strain OL1, DSMZ 11500). The isolates showed special adaptation...

Due to contamination of barley grains by Fusarium langsethiae, T-2 toxin can be present in the brewing process. It has been observed that the presence of the yeast Geotrichum candidum during malting can reduce the final concentration of this mycotoxin in beer. In this work, a co-culture method was carried out for both microorganisms in order to evaluate the effect on T-2 mycotoxin concentration...

today, in times of globalisation, intercultural competence is required more than ever before. therefore, language institutes are advocating and keen to train their students in intercultural competence too. accordingly, they ensure that their teachers include teaching additional cultural information about the country of the language being taught in their courses. the fact is, however, that durin...

conclusions the results prove that the presented double pcr method could be used to detect positive cases from culture-negative blood samples. the chelex 100 method is simpler and safer than the use of qiagen kit for dna extraction. materials and methods a total of 100 patients with suspected brucellosis were included in this experimental study and given positive serological tests. diagnosis wa...

conclusions this paper may serve as a guide to screening, isolation, and cultivation of more new mtb. the new isolated strain opens up good opportunities for biotechnological applications such as medicine to bioremediation processes due to its unique abilities. results the results revealed a correlation of important physicochemical factors such as ph and iron with growth and blooms of these bac...