Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat. Here we report a 110-Mb draft sequence of Pst isolate CY32, obtained using a 'fosmid-to-fosmid' strategy, to better understand its race evolution and pathogenesis. The Pst genome is highly heterozygous and contains 25,288 protein-coding genes. Compared with non-obligate fungal pat...

Detecting the presence and amount of a fungus in leaf tissue, especially when it is suspected that other fungi are presen~, is quite difficult. Culture methods can be unreliable because a few spores of a fast growing fungus may overgrow other fungi within a, few days (e.g. McKENZIE and HUDSON 1976). Methods which measure the chitin content of fungal walls cannot differentiate between fungi; in ...

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Verticillium lecanii has been found growing in pustu'les of the uredial stage of a number of rust fungi (GAMS 1971, SCHROEDER and HAssEBRAuK 1957, CASPER and MENDGEN 1979, SPENCER 1'980), and in some insects (HALL 1980). SCHROEDER and HASSEBRi\UK (1957) observed that this fungus is able to penetrate the urediospores of Puccinia striiformis. It remains to be shown, however, whether Verticillium ...