Metarhizium anisopliae is a fungus of considerable metabolic and ecological versatility, being a potent insect pathogen that can also colonize plant roots. The mechanistic details of these interactions are unresolved. We provide evidence that M. anisopliae adheres to insects and plants using two different proteins, MAD1 and MAD2, that are differentially induced in insect hemolymph and plant roo...

Bioassays were conducted to document the effects of Metarhizium anisopliae infection on adult female Asian longhorned beetle (Anoplophora glabripennis) reproduction before death and subsequent survival of offspring. The effect of infection on fecundity was evaluated for females already laying eggs and for newly eclosed females using M. anisopliae isolates ARSEF 7234 and 7711, respectively. Decr...

Ambient pH regulates the expression of virulence genes of Metarhizium anisopliae, but it was unknown if M. anisopliae can regulate ambient pH. Mutants of M. anisopliae altered in production of oxalic acid were evaluated for the interrelationship of ambient pH, buffering capacity added to media, growth, and generation of extracellular proteases and ammonia. Wild-type and acid-overproducing mutan...

The exopolysaccharide (EPS) separated from the entomopathogenic fungus Metarhizium anisopliae was determined by gel permeation chromatography to be homogeneous. The high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAE-PAD) showed a content of monosaccharides D-galactosamine and D-fucose at a molar ratio of about 2:1. The results obtained from Fourier transform...

in the present study, pathogenicity of four native strains of entomopathogenic fungus; metarhizium anisopliae, was studied against adult stage of ixodes ricinus. for this purpose a total number of 180 adult ticks were examined in triplicate. thirty ticks for each strain and negative and positive controls were immersed in 2.4×107 fungal conidia/ml in vitro. samples were incubated in separate pet...

Bed bugs Cimex lectularius L. were exposed to conidia (spores) of the entomopathogenic fungus Metarhizium anisopliae by feeding, aerosol spray, or contact with a treated surface. Feeding experiments demonstrated that bed bugs were innately susceptible to this fungus. However, only at 98% humidity were mortality rates high, regardless of whether bed bugs were sprayed with a fungal solution or co...

We present two cases of keratitis due to Metarhizium anisopliae in geographically separated areas of the United States. The isolates were microscopically similar but morphologically different and were identified by ribosomal DNA sequencing. Both isolates had low minimum inhibitory concentration (MIC) values to caspofungin and micafungin, but high MIC values to amphotericin B. The morphologic an...

Mortality of rice weevil, Sitophilus oryzae (L.) caused by Metarhizium anisopliae (Metschnikoff) Sorokin alone and in combination with a neonicotinoid insecticide, acetamiprid was evaluated in laboratory bioassays. Spray application of M. anisopliae alone at a spore concentration of 6 x 10 conidia/ml required 6 days to cause 10 percent mortality whereas 4 x 10 conidia/ml required 8 days to caus...

Entomopathogenic fungi are naturally occurring pathogens of some insects that produce enzymes capable of penetrating into an insect’s cuticle. Chitinases are thought to be responsible for entomopathogenic fungi’s ability to degrade cuticle. Primers were designed for the chi3 gene which encodes a chitinase CHIT30, from the mRNA transcript present in Metarhizium anisopliae. These primers were una...

Cecropins are the most potent induced peptides to resist invading microorganisms. In the present study, two full length cDNA encoding cecropin2 (Px-cec2) and cecropin3 (Px-cec3) were obtained from P. xylostella by integrated analysis of genome and transcriptome data. qRT-PCR analysis revealed the high levels of transcripts of Px-cecs (Px-cec1, Px-cec2 and Px-cec3) in epidermis, fat body and hem...