Promoter sequences of the cytomegalovirus (PCMV), the rous sarcoma virus long terminal repeat (PRSV-LTR) and the cauliflower mosaic virus 35s (PCaMV35s) were ligated with the beta-glucuronidase (GUS) gene, uidA, and were introduced into cells of the marine diatom, Phaeodactylum tricornutum. Transformants were selected on a 100 mg l(-1) Zeocin plate, and Zeocin-resistant clones were further sele...

INTRODUCTIONThe MS2 system provides optimal sensitivity for single-molecule detection in cells. It requires two genetically encoded moieties: a reporter mRNA that contains MS2 binding site (MBS) stem loops and a fluorescent MS2 coat protein (MCP-xFP) that binds to the stem loops with high affinity, thus tagging the mRNA within the cell. This protocol describes transfection of COS-7 cells with r...

In this work, five different thermodynamic models for correlating the fugacity of water and Tetrahydrofuran (THF) in liquid phase in equilibrium with hydrogen hydrate phase, based on the van der Waals–Platteeuw (vdW-P) statistical thermodynamic model are used and the equilibrium pressures of hydrogen hydrate at different temperatures are calculated. The dissociation pressure of binary hydrogen ...