In this study, the immunogenicity of three types of antigens (hydatid fluid, protoscolices and whole body of E. granulosus) was investigated in lambs by ELISA. Sixteen 4–6-month-old lambs of mixed sexes were divided into 4 groups of 4 lambs (three immunized and one control group). Twelve lambs as immunized groups received 2 mg of hydatid fluid, protoscolices and whole body of E. granulosus ant...

Full-length cDNA and genomic DNA encoding an 8-kDa subunit of antigen B from Echinococcus multilocularis (designated EmAgB8/1) were isolated from an E. multilocularis metacestode cDNA library and a protoscolex genomic DNA library, respectively. The open reading frame of the cDNA clone encodes a polypeptide comprising 85 amino acids with a 20-amino-acid NH(2)-terminal signal sequence, which was ...

A 22-year-old man with end-stage renal disease on haemodialysis (HD) was admitted to our hospital with a generalized abdominal pain. He had been on HD without complications the previous year. One year before admission, peritoneal dialysis was discontinued because of peritonitis due to candida infection. At physical examination, his blood pressure was 150/90mmHg and heart rate was 90 b.p.m. His ...

An increasing number of cases of echinococcosis in Thailand have been imported, probably native infections and medical transfers. Serodiagnosis is one diagnostic choice for interpreting infections before a further step is done. Due to limited antigen, indirect ELISA has been used as a negative screening test for IgG-detection to rule out echinococcosis. Native hydatid cystic fluid (HCF) antigen...

background: our objectives were to investigate whether immunomodulatory cytokines, tgf-β and il-10, are stimulated in response to cystic echinococcosis (ce) components in mice model, and whether major hydatid fluid antigens or live protoscoleces could equally contribute to such cytokines. methods: protoscoleces were obtained by aseptic puncture of fertile sheep hydatid cysts. hydatid fluid anti...

An IgM murine monoclonal antibody (MAb) was obtained against the excretory-secretory antigen (ES-Ag) of in vitro reared protoscoleces of Echinococcus granulosus (Batsch, 1786). Western blotting revealed that the MAb recognised a 20.6 kDa protein of this ES-Ag. The MAb was used in sandwich enzyme-linked immunosorbent assay (s-ELISA) for selective sensitisation of the solid phase with the protosc...

Cystic echinococcosis (CE) is a worldwide distributed helminthic zoonosis caused by Echinococcus granulosus. Benzimidazole derivatives are currently the only drugs for chemotherapeutic treatment of CE. However, their low efficacy and the adverse effects encourage the search for new therapeutic targets. We evaluated the in vitro efficacy of Bortezomib (Bz), a proteasome inhibitor, in the larval ...

In current study, a few sheep cystic livers and lungs were obtained from Mashhad slaughter house and protoscolex were separated aseptically. Thirty 6-week-old Swiss mice were divided into 5 groups and two thousands of live protoscolices were inoculated intrapritoaneally in each mouse. In group 1 and 2 (prophylactic groups), mice were given 150 mg/kg of oral albendazole and mebendazole for 10 da...