Allium cepa, Guard Cell Protoplasts, Malate, Vicia faba Isolated and purified guard cell protoplasts of starch-containing ( Vicia faba) and starch-lacking (Allium cepa) stomata were used to study the metabolism of malate and Clanions as correlated with the K +-induced swelling of protoplasts. The correlation between swelling and malate synthesis in starch-containing and the inhibition of this c...

it is not always easy to prepare healthy, viable ones, and also it is not easy to measure the kinetics of their changing A quick and accurate method of monitoring changes dimensions in response to an osmotic gradient. Recent of volume of mesophyll cell protoplasts (MCP) of pea studies used vesicles prepared from root cells of wheat was devised using the difference in absorbance of the (Niemietz...

Possibilities to account for the mechanism of freeze-thaw injury to isolated protoplasts of Spinacia oleracea L. cv. Winter Bloomsdale were investigated. A freeze-thaw cycle to -3.9 C resulted in 80% lysis of the protoplasts. At -3.9 C, protoplasts are exposed to the equivalent of a 2.1 osmolal solution. Isolated protoplasts behave as ideal osmometers in the range of concentrations tested (0.35...

The enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase displayed near-maximal activity in isolated, intact barley (Hordeum vulgare L. cv. Pennrad) mesophyll protoplasts. The carboxylase deactivated 40 to 50% in situ when protoplasts were dark-incubated 20 minutes in air-equilibrated solutions. Enzyme activity was fully restored after 1 to 2 minutes of light. Addition of 5 millimolar NaHCO(3...

The time of appearance and relative amounts of alpha-amylase mRNA in wild oat (Avena fatua L.) aleurone protoplasts incubated with 1 micromolar gibberellin A(4) (GA(4)) were closely correlated with the amounts of alpha-amylase enzyme secreted by the protoplasts. In the absence of GA(4), or when protoplasts were incubated with 25 micromolar abscisic acid (ABA) together with 1 micromolar GA(4) no...

Multinucleate soybean protoplasts produced by spontaneous fusion during enzyme digestion of the cell wall initiated cell division after approximately 40 h in culture. The structure of these protoplasts during mitosis and cytokinesis was studied with both light and electron microscopes. Most nuclei did not fuse but divided synchronously. Interphase nuclei was commonly connected by short narrow n...

We demonstrated that the liposomes composed of dioleolylphosphatidylethanolamine/cholesterol/oleic acid (4:4:2) dramatically release their contents at a pH of less than or equal to 6.0 and are capable of delivering their contents into the cytoplasm of higher plant protoplasts. This is shown by using a soluble fluorescent dye, calcein, as a liposome-entrapped marker. We found that calcein fluore...

The inhibitory allelopathic activities of leaves and leaf-origin suspension cultured cells Spiraea thunbergii, putative allelochemicals, cis- trans-cinnamic acid, were investigated by the protoplast co-culture method with digital image analysis (DIA-PP method) using lettuce as a recipient. optimal conditions 2,4-dichlorophenoxyacetic acid (2,4-D) benzyladenine (BA) for cell division S. thunberg...

Cannabis sativa L. is a valuable, up-and-coming industrial crop with substantially growing market. However, due to an extended period of legal restriction, research cannabis has been limited, particularly in laboratory settings. Expanding the application biotechnological techniques can facilitate addressing species-specific impediments improving traits and further fundamental understanding its ...

The present study reports an efficient protocol for isolation and regeneration of protoplasts from callus of Fritillaria imperialis L. There is no published method recommended for protoplast isolation and regeneration from Fritillaria imperialis L. A range of factors, which influence the success of isolation and regeneration of F. imperialis protoplasts, were investigated. From the results obta...