RNA and protein expression profiling technologies have revolutionized how toxicologists can study the molecular basis of adverse effects of chemicals and drugs. It is expected that these new technologies will afford efficient and high-throughput means to delineate mechanisms of action and predict toxicity of unknown agents. To reach these goals, a more thorough understanding of the constraints ...

The neuromuscular junction (NMJ) plays a fundamental role in transferring information from lower motor neuron to skeletal muscle to generate movement. It is also an experimentally accessible model synapse routinely studied in animal models to explore fundamental aspects of synaptic form and function. Here, we combined morphological techniques, super-resolution imaging, and proteomic profiling t...

In proteomic experiments quantification becomes increasingly important not only for differential proteomic analysis, but also for the identification of specific proteins studying protein complexes (e. g. by affinity purification [1]). Thus, due to increasing sensitivity and scan speed of modern MS instruments, targets and controls show largely overlapping protein patterns with some proteins pre...

This paper focuses on the application of proteomic tools to study the composition and natural history of snake venoms, and their crossreactivity with current homologous and heterologous antivenoms. Proteomic analyses on Bothrops indicated the suitability of using PLA2 molecules as taxonomic and population-specific markers. The lack of phylogenetic clustering among Neotropical and Neartic rattle...

Plant drought tolerance is a complex trait that requires a global view to understand its underlying mechanism. The proteomic aspects of plant drought response have been extensively investigated in model plants, crops and wood plants. In this review, we summarize recent proteomic studies on drought response in leaves to reveal the common and specialized drought-responsive mechanisms in different...

Cellular mechanisms of stress sensing and signaling represent the initial plant responses to adverse conditions. The development of high-throughput "Omics" techniques has initiated a new era of the study of plant molecular strategies for adapting to environmental changes. However, the elucidation of stress adaptation mechanisms in plants requires the accurate isolation and characterization of s...

background: gliomas are the most frequently observed primary brain tumors. these tumors comprise a variety of different histological tumor types and malignancy grades. oligodendrogliomas typically contain a rich network of branching capillaries. approximately 50%-80% of oligodendrogliomas demonstrate a combined loss of chromosomes 1p and 19q. oligodendrogliomas differ from neurocytomas in that ...

to better understand seed priming that improve seed germination and seedling uniformity, we examined the physiological role of osmopriming in germination of bread wheat (triticum aestivum l.) seed embryos by a proteomic approach. for this purpose, we used two systems. the first system consisted of osmo-primed seeds by peg 6000 at -0.8 mpa at 15?c for 24 hours, followed by air drying at 15?c, an...

BACKGROUND Preterm Birth (PTB) is a major cause of neonatal mortality and morbidity. Women with Polycystic Ovary Syndrome (PCOS) are at high risk of PTB. There is a need for research studies to investigate the mechanisms linking PCOS and PTB, to facilitate screening, and develop novel preventative strategies. OBJECTIVE To list all the proteomic biomarkers of PTB and integrate this list with t...

OBJECTIVE Pathological changes in an organ or tissue may be reflected in proteomic patterns in serum. It is possible that unique serum proteomic patterns could be used to discriminate cancer samples from non-cancer ones. Due to the complexity of proteomic profiling, a higher order analysis such as data mining is needed to uncover the differences in complex proteomic patterns. The objectives of ...