Antineutrophil cytoplasmic antibodies (ANCA) are predominantly IgG autoantibodies directed against constituents of primary granules of neutrophils and monocytes lysosomes. Although several antigenic targets have been identified, those ANCA directed to proteinase 3 or myeloperoxidase are clinically relevant, whereas the importance of other ANCA remains unknown. Both are strongly associated with ...

Cell proliferation has been found to correlate with increased secretion of proteinases, such as plasminogen activator, in several different cell populations. In addition, the shape of the cell may also play a role in regulating proteinase secretion. However, the relationship between cell proliferation, cell shape and proteinase secretion has not been studied in diploid epithelial (E) cells cult...

Three proteinases which digest hemoglobin rapidly at acid pH (3.5 to 4.5) were identified in crude extracts of soybean (Merr.) leaves and separated by chromatography on DEAE-cellulose. All three enzymes were endopeptidases as judged by the ratio of alpha-amino-nitrogen plus peptide nitrogen over alpha-amino-nitrogen in the trichloroacetic acid-soluble portion of hemoglobin digests. Proteinase I...

1. Lysosomal proteinase activity was assayed in human cadaver kidney, urine, granules from poly­ morphonuclear leucocytes of normal persons, and urine samples from 154 patients with renal disease. 2. Granules from polymorphonuclear leuco­ cytes showed proteinase activity at acid and neutral pH, whereas cadaver kidney showed proteinase activity at acid pH only. 3. The urine from 13 patients with...

Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chloroform; a plant proteinase (E6870) followed by phenol-chloroform; and boiling of leptospires in 0.1 mM Tris, pH 7.0 for 10 min at 100 degrees C, with no phenol treatment. Every strain treated with proteinase K or E6870 afforded positive polymerase chain reaction (PCR) reaction. On the other hand, from...

Human immunodeficiency virus type 1 expresses structural proteins and replicative enzymes within gag and gag-pol precursor polyproteins. Specific proteolytic processing of the precursors by the viral proteinase is essential for maturation of infectious viral particles. We have studied the activity of proteinase in its immature form, as part of a gag-pol fusion protein, in an in vitro expression...

Slime and proteinase activity of 54 strains consisting of 19 Candida parapsilosis and 35 C. albicans strains isolated from blood samples were investigated in this study. Ketoconazole, amphothericin B, and fluconazole susceptibility of Candida species were compared with slime production and proteinase activity of these species. For both Candida species, no correlation was detected between the sl...

An attempt was made to substantiate a modori (thermal gel degradation) inducing effect of a latent serine proteinase. The proteinase was purified homogeneously from the sarcoplasmic fraction of threadfin bream muscle using a combination of DEAF-cellulose, Con A-Sepharose, ArgSepharose, Shim-pack HAC, and TSK-gel G3000SWXL column chromatographies. Its molecular weight was estimated as 77,000 by ...

The interaction of copper oxide nanoparticles and copper ions and proteinase K from Tritirachium album Limber has been investigated employing UV spectroscopy and kinetics measurements. The aim of this study was to evaluate the effect of nanoparticles of CuO for proteinase K. In this paper we compare the effect of copper oxide nanoparticles with the effect of copper ions on proteinase K stabilit...